外泌体hsa-miR-522-3p通过调节CDK6参与卵巢癌进程

Exosome hsa-miR-522-3p is involved in ovarian cancer progression by regulating CDK6

目的:探讨外泌体hsa-miR-522-3p在卵巢癌发生发展中的作用及其潜在机制。方法:采用荧光实时定量PCR检测hsa-miR-522-3p在组织和细胞中的表达水平。使用EdU、Transwell和流式细胞术检测外泌体hsa-miR-522-3p对卵巢癌细胞增殖、迁移、侵袭和凋亡的影响。通过荧光素酶测定和蛋白质印迹评估hsa-miR-522-3p对CDK6表达的调控作用。进一步构建裸鼠成瘤模型验证外泌体hsa-miR-522-3p对肿瘤生长的影响。结果:本研究发现hsa-miR-522-3p在卵巢癌组织、血浆外泌体及卵巢癌细胞的外泌体中表达显著升高。与NC组比较,hsa-miR-522-3p模拟物组细胞的迁移和侵袭能力显著增高且差异具有统计学意义(P<0.05)。此外,hsa-miR-522-3p抑制剂组可以促进caspase3和Bax的表达并抑制Bcl2的表达。荧光素酶报告结果显示,hsa-miR-522-3p能够靶向结合CDK6的3′-非翻译区(3′-UTR)来抑制其表达。裸鼠成瘤结果表明,外泌体hsa-miR-522-3p可增加瘤的体积及重量。结论:外泌体hsa-miR-522-3p通过调节CDK6参与卵巢癌的进程。本研究的发现将为外泌体hsa-miR-522-3p在卵巢癌发生中的机制提供新的见解。

Objective:To explore the role of exosome hsa-miR-522-3p in the occurrence and development of ovarian cancer and its potential mechanism.Methods:Real time quantitative PCR was used to detect the expression level of hsa-miR-522-3p in tissues and cells.The effects of extracellular vesicles and hsa-miR-522-3p on cell proliferation,migration,invasion,and apoptosis were detected using EdU,Transwell,and flow cytometry.Evaluate the regulatory effect of hsa-miR-522-3p on CDK6 expression through luciferase assay and Western blotting.Further construct a nude mouse tumor formation model to verify the effect of exosomes hsa-miR-522-3p on tumor growth.Results:This study found that hsa-miR-522-3p was significantly increased in ovarian cancer tissue,plasma exosomes,and exosomes of ovarian cancer cells.Compared with the NC group,the migration and invasion ability of cells in the hsa-miR-522-3p group was significantly increased,and the differences were statistically significant(P<0.05).In addition,the hsa-miR-522-3p inhibition group can promote the expression of caspase3 and Bax and inhibit the expression of Bcl2.The results of double luciferase report showed that hsa-miR-522-3p could target the 3'-untranslated region(3'-UTR)of CDK6 to inhibit its expression.The results of tumor formation in nude mice indicate that the exosomes hsa-miR-522-3p can increase the volume and weight of the tumor.Conclusion:Exosomes hsa-miR-522-3p participate in the progression of ovarian cancer by regulating CDK6.The findings of this study will provide new insights into the mechanism of exosomes hsa-miR-522-3p in the development of ovarian cancer.