SCF/c-kit信号通路在肝内胆汁淤积症中与颗粒细胞凋亡的关系

Relationship between SCF/c-kit Signaling Pathway and Granulosa Cell Apoptosis in Intrahepatic Cholestasis

目的:探讨血清干细胞因子(SCF)/酪氨酸蛋白激酶生长因子受体(c-kit)信号通路在肝内胆汁淤积症中与颗粒细胞凋亡的关系。方法:将黑龙江省医院普外三科2017年3月—2020年12月收治的肝内胆汁淤积症患者41例纳入研究,记作病变组,另取同期健康受试者41例作为健康组。分别比较两组颗粒细胞凋亡情况,血清SCF、c-kit表达水平,颗粒细胞内Bcl-2及Caspase-3 mRNA转录水平,并作相关性分析。结果:病变组颗粒细胞凋亡细胞DNA含量、血清SCF、c-kit表达水平、Caspase-3 mRNA转录水平均高于健康组(P<0.05),病变组颗粒细胞内Bcl-2 mRNA转录水平低于健康组(P<0.05)。经Pearson相关性分析可得:肝内胆汁淤积症患者血清SCF、c-kit表达水平与颗粒细胞内Bcl-2 mRNA转录水平呈负相关(r=-0.532、-0.583,P=0.001、0.000),而与Caspase-3 mRNA转录水平呈正相关(r=0.479、0.510,P=0.001、0.004)。结论:在肝内胆汁淤积症中,SCF/c-kit信号通路可能和颗粒细胞凋亡密切相关,两者之间的联系可能是通过Bcl-2、Caspase-3的表达实现。

Objective:To investigate the relationship between serum stem cell factor(SCF)/tyrosine protein kinase growth factor receptor(c-kit)signaling pathway and granulosa cell apoptosis in intrahepatic cholestasis.Method:A total of 41 patients with intrahepatic cholestasis admitted to the Third Department of General Surgery of Heilongjiang Provincial Hospital from March 2017 to December 2020 were included in the study and recorded as the diseased group,and 41 healthy subjects in the same period were selected as the healthy group.The apoptosis of granulosa cells,the expression levels of serum SCF and c-kit,the mRNA transcription levels of Bcl-2 and Caspase-3 in granulosa cells were compared between the two groups,and the correlation was analyzed.Result:The DNA content of apoptotic granulosa cells,expression levels of serum SCF,c-kit and the mRNA transcription of Caspase-3 in the diseased group were higher than those in the healthy group(P<0.05),the mRNA transcription level of Bcl-2 in granulosa cells of the diseased group was lower than those of the healthy group(P<0.05).Pearson correlation analysis showed that the expression levels of SCF and c-kit in serum of patients with intrahepatic cholestasis were negatively correlated with the transcription level of Bcl-2 mRNA in granular cells(r=-0.532,-0.583;P=0.001,0.000),but positively correlated with Caspase-3 mRNA transcription level(r=0.479,0.510;P=0.001,0.004).Conclusion:SCF/c-kit signaling pathway may be closely related to granulosa cell apoptosis in intrahepatic cholestasis,and the relationship between them may be mediated by the expression of Bcl-2 and Caspase-3.