生防放线菌ZZ-9颗粒剂的研制及对黄瓜立枯病的防治效果

Research of Biocontrol Actinomycete ZZ-9 Granules and Its Effect on Cucumber Rhizoctonia Rot

娄彻氏链霉菌Streptomyces rochei ZZ-9是一株有广谱抑菌活性的生防菌株。本研究采用菌丝生长速率法,通过对载体、稳定剂、崩解剂、粘结剂、湿润剂的筛选,确定娄彻氏链霉菌ZZ-9颗粒制剂的最佳配方:菌液与凹凸棒土比例为2:5,碳酸钙1%,氯化镁2%,聚乙烯醇1%,十二烷基苯磺酸钠0.5%。试验结果显示制备的ZZ-9颗粒剂有效活菌数为1.19×10^(8)cfu/g,粒度为93.5%,水分含量2.5%,水中崩解时间96 s,pH值6.35,硬度为10%。盆栽防病试验结果表明,制备的ZZ-9颗粒剂在施用量为3 g/盆时,对黄瓜立枯病的防治果最佳达到76.92%。对黄瓜幼苗生长的促生作用最明显,发芽率、株高、根长和鲜重分别较对照提高了9.34%、29.52%、27.54%、22.96%。

Streptomyces rochei ZZ-9 is a biocontrol strain that with broad-spectrum antifungal activity.The mycelium growth rate method was utilized in this work to screen carriers,stabilizers,disintegrants,adhesives,and wetting agents.The optimal formula of Streptomyces rochei ZZ-9 granules were:the ratio of bacterial solution and attapulgite was 2:5,calcium carbonate 1%,magnesium chloride 2%,polyvinyl alcohol 1%,sodium dodecylbenzene sulfonate 0.5%.The effective viable number of ZZ-9,particle size,moisture content,disintegration time in water,pH value and hardness were 1.19×10^(8) cfu/g,93.5%,2.5%,96 s,6.35 and 10%.The results of pot experiment showed that the optimal controlling effect of cucumber Rhizoctonia rot was 76.92%after application of ZZ-9 granule with 3 g/pot.In addition,the Streptomyces rochei ZZ-9 granules presented significant promoting effects in pot experiment.Compared with the control,the germination rate,plant height,root length and fresh weight of cucumber seedlings were increased by 9.34%,29.52%,27.54%and 22.96% after application of Streptomyces rochei ZZ-9 granules,respectively.