云芝胞内糖肽的糖类含量测定和指纹图谱研究

Study on Content Determination and Chromatographic Fingerprints of Sugars in Intracellular Glycopeptide of Coriolus Versicolor

目的建立云芝胞内糖肽的游离糖和水解单糖指纹图谱和含量测定的检测方法。方法游离糖测定以水为溶剂超声提取后PMP衍生化,水解单糖测定酸水解后PMP衍生化,采用HPLC对来自不同企业的云芝胞内糖肽原料、中间体的游离糖和水解单糖进行定性和定量分析。通过化学计量学分析对指纹图谱进行了分析,探讨不同生产企业生产的云芝胞内糖肽差异性。结果2种方法均通过方法学验证;不同企业的样品分别聚类,有3种游离糖和2种水解单糖对云芝胞内糖肽的质量差异贡献较大。结论建立的分析方法可有效用于云芝胞内糖肽中游离糖和水解后的单糖的测定和指纹图谱研究,采用的化学计量学研究方法对云芝胞内糖肽的质量控制提供了指导意义。

OBJECTIVE To establish a detection method for fingerprints and content determination of free sugar and hydrolyzed monosaccharide of intracellular glycopeptides in Coriolus versicolor.METHODS PMP derivatization after ultrasonic extraction with water as solvent was determined for free sugars,and PMP derivatization after acid hydrolysis was determined for hydrolyzed monosaccharides.The free sugars and hydrolyzed monosaccharides of raw materials and intermediates of intracellular glycopeptides from different manufacturers were analyzed by HPLC qualitatively and quantitatively.The fingerprints were analyzed by chemometrics analysis,and the differences between intracellular glycopeptides produced by different manufacturers were discussed.RESULTS Both two methods were validated by methodology.The samples from different manufacturers were clustered separately,and three free sugars and two hydrolyzed monosaccharides contributed greatly to the quality difference of intracellular glycopeptides of Coriolus versicolor.CONCLUSION The established analytical method can be effectively used for the determination of free sugars and hydrolyzed monosaccharides in the intracellular glycopeptide of Coriolus versicolor and the study of fingerprints.The adopted chemometrics research method provides guidance for the quality control of the intracellular glycopeptide of Coriolus versicolor.