iRoot BP Plus与TheraCal LC对人牙髓细胞体外增殖分化的影响比较

Effect of iRoot BP Plus and TheraCal LC on proliferation and odontogenic differentiation of human dental pulp cells in vitro

目的:比较iRoot BP Plus和TheraCal LC对人牙髓细胞(human dental pulp cells,hDPCs)体外增殖分化的影响。方法:培养hDPCs,CCK-8法检测不同质量浓度iRoot BP Plus、TheraCal LC对hDPCs增殖的影响,确定最佳干预质量浓度。以最佳浓度培养hDPCs 7 d、14 d后,检测碱性磷酸酶(alkaline phosphatase,ALP)活性;培养2 d、4 d后检测成牙本质相关基因的表达。结果:iRoot BP Plus和TheraCal LC均能显著促进hDPCs的增殖、ALP活性以及成牙本质相关基因的表达,但两者之间并无明显统计学差异。结论:TheraCal LC促进hDPCs增殖及成牙本质分化的效果与iRoot BP Plus相当,值得临床推荐使用。

Objective:To compare the effect of iRoot BP Plus and TheraCal LC on proliferation and odontogenic differentiation of human dental pulp cells(hDPCs)in vitro.Methods:hDPCs were isolated and cultured,the effect of iRoot BP Plus and TheraCal LC on the proliferation of hDPCs were evaluated by CCK-8 assay to determine the optimal concentration of intervention quality.ALP activity at time-point day 7 and day 14 were determined.The expression of odontogenesis related genes were tested on days 2 and 4.Results:The proliferation,ALP activity and expression of odontogenesis related genes of hDPCs were significantly increased by both iRoot BP Plus and TheraCal LC.But there was no significant difference between these two substances.Conclusion:The effect of TheraCal LC on proliferation and mineralization of hDPCs was similar to iRoot BP Plus,iRoot BP Plus and TheraCal LC were both worthy of clinical application.