红景天苷对MCAO大鼠脑血管内皮细胞的作用

Effects of salidroside on cerebral vascular endothelial cells in MCAO rats

目的研究红景天苷(salidroside,SAL)对缺血性脑损伤大鼠脑血管内皮细胞的作用及其作用机制。方法健康成年SD雄性大鼠24只,线栓法制备MCAO模型,随机分为假手术组(Sham)、模型组(MCAO)、SAL给药组(MCAO+SAL),SAL给药浓度为50 mg·kg^(-1),连续给药6 d。Western blot检测大鼠损伤侧脑组织ICAM-1、VCAM-1、E-selectin、P-selectin的蛋白表达。体外实验采用HUVECs,经不同浓度SAL(0.1、1、10μmol·L^(-1))和LPS(100μg·L^(-1))分别干预24 h,CCK-8法检测SAL和LPS对HUVECs存活的影响;体外血管形成实验中LPS组(100μg·L^(-1))和SAL给药组(LPS+SAL)干预HUVECs 24 h,SAL给药浓度分别为0.1、1、10μmol·L^(-1),观察LPS和SAL对其血管形成的影响。Western blot检测HUVECs的ICAM-1、VCAM-1、E-selectin、P-selectin蛋白表达。结果SAL可以降低MCAO大鼠损伤侧脑组织中ICAM-1、VCAM-1、E-selectin、P-selectin表达;体外研究发现,SAL对HUVECs存活无影响;LPS抑制HUVECs的血管形成,经SAL作用后,SAL(1、10μmol·L^(-1))逆转LPS的作用,促进其血管生成;与对照组(Control)相比,LPS促进HUVECs中ICAM-1、VCAM-1、E-selectin、P-selectin蛋白表达,而经SAL作用后,ICAM-1、VCAM-1、E-selectin、P-selectin表达量明显降低,促进HUVECs血管形成能力。结论SAL具有改善缺血性脑损伤大鼠内皮细胞的再生能力,以及促进血管形成能力。

Aim To study the effect of salidroside(SAL)on cerebral vascular endothelial cells of rats with ischemic brain injury and its mechanism of action.Methods Twenty-four healthy adult SD male rats were prepared by bolt plugging method to prepare MCAO models,and randomly divided into sham surgery group(Sham),model group(MCAO),and SAL administration group(MCAO+SAL),and the concentration of SAL was 50 mg·kg^(-1),with a continuous administration for six days.Western blot was used to detect the protein expression of ICAM-1,VCAM-1,E-selectin,and P-selectin in injured brain tissue of rats.In vitro cell experiments using HUVECs were subjected to different concentrations of salidroside(0.1,1,10μmol·L^(-1))and LPS(100μg·L^(-1))intervened for 24 hours,and CCK-8 was employed to detect the effects of SAL and LPS on the survival of HUVECs.In vitro angiogenesis experiments,LPS group(100μg·L^(-1))and SAL administration group(LPS+Sal)intervened in HUVECs for 24 hours,and the concentrations of SAL administration were 0.1,1,and 10μmol·L^(-1),then the effects of LPS and SAL on their angiogenesis were observed.The protein expressions of ICAM-1,VCAM-1,E-selectin,and P-selectin in HUVECs were detected by Western blot.Results SAL could reduce the expression of ICAM-1,VCAM-1,E-selectin,and P-selectin in ischemic brain tissue of MCAO rats.In vitro experimental studies found that salidroside had no effect on the survival of HUVECs.LPS inhibited the angiogenesis of HUVECs,and after the action of SAL,SAL(1,10μmol·L^(-1))reversed the effect of LPS and promoted its angiogenesis.Compared with the control group,the expressions of ICAM-1,VCAM-1,E-selectin and P-selectin of HUVECs after LPS stimulation increased,while the expressions of ICAM-1,VCAM-1,E-selectin and P-selectin were significantly reduced after the addition of SAL,which promoted the angiogenesis ability of HUVECs.Conclusions SAL can improve the ability of cell regeneration in rats with ischemic brain injury and promote the ability of blood vessel formation.