外源褪黑素对干旱胁迫下沙芦草幼苗生长和生理特性的影响

Effects of exogenous melatonin on growth and physiological characteristics of Agropyron mongolicum seedlings under drought stress

为明确外源褪黑素对沙芦草干旱胁迫的缓解效应,以‘盐池’沙芦草为试材,采用聚乙二醇6000(PEG-6000)模拟干旱胁迫,研究沙芦草幼苗对干旱胁迫的响应以及外源添加不同浓度(0、1、10、50、100、150和200 mg·L^(-1))褪黑素对干旱胁迫下沙芦草幼苗生长和生理特性的影响。结果表明:干旱胁迫显著抑制沙芦草幼苗的生长,而外源添加不同浓度的褪黑素均能够缓解干旱胁迫引起的沙芦草幼苗生长抑制,并且当褪黑素浓度为100 mg·L^(-1)时缓解效果最显著。与单独干旱胁迫处理相比,在干旱胁迫下外源添加100 mg·L^(-1)褪黑素后沙芦草幼苗株高、地上干重和叶片相对含水量分别增加58.2%、121.2%和48.1%,叶绿素a、叶绿素b和类胡萝卜素含量分别增加48.7%、80.8%和38.3%,超氧化物歧化酶、过氧化物酶和根系活力分别增加12.6%、33.9%和39.1%,抗坏血酸和谷胱甘肽含量分别增加19.5%和18.3%,脯氨酸、可溶性糖和可溶性蛋白含量分别提高16.2%、32.6%和14.3%,而丙二醛、过氧化氢和超氧阴离子含量分别降低45.8%、65.8%和30.8%。综上,外源添加100 mg·L^(-1)褪黑素能够显著促进沙芦草幼苗生长,增强抗氧化能力,增加渗透调节物质含量,抑制干旱胁迫下沙芦草植株体内活性氧的过度产生,降低膜质过氧化水平,从而提高沙芦草幼苗的耐旱性。

To clarify the alleviation effect of exogenous melatonin(MT)on Agropyron mongolicum under drought stress,we examined the response of A.mongolicum‘Yanchi'seedlings to simulated drought stress with polyethylene glycol 6000(PEG-6000),by investigating the effects of exogenous addition of different concentrations(0,1,10,50,100,150 and 200 mg·L~(-1))of MT on seedlings growth and physiological characteristics under drought stress.The results showed that drought stress significantly inhibited the growth of A.mongolicum seedlings,and that exogenous addition of different concentrations of MT could alleviate the growth inhibition caused by drought stress,with the strongest mitigation effect observed at MT concentration of 100 mg·L~(-1).Compared with the drought stress treatment alone,exogenous addition of 100 mg·L~(-1)MT under drought stress increased plant height,aboveground dry weight,and leaf relative water content by 58.2%,121.2%and 48.1%.The contents of chlorophyll a,chlorophyll b,carotenoids increased by 48.7%,80.8%and 38.3%,superoxide dismutase,peroxidase and root activity increased by 12.6%,33.9%and 39.1%,and the contents of ascorbic acid and glutathione increased by 19.5%and 18.3%,respectively.The contents of proline,soluble sugar and soluble protein were increased by 16.2%,32.6%and 14.3%,while that of malondialdehyde,hydrogen peroxide and superoxide anion radical were decreased by 45.8%,65.8%and 30.8%,respectively.In summary,exogenous addition of 100 mg·L~(-1)MT could improve drought tolerance of A.mongolicum seedlings by promoting growth,enhancing antioxidant capacity,increasing the content of osmoregulation substances,inhibiting the excessive production of reactive oxygen,and reducing membrane peroxide level.