HPLC测定毛葡萄汁和桑椹汁及毛葡萄醋和桑椹醋中18种酚类物质

Determination of 18 Phenolics in Vitis quinquangularis Juice and Mulberry Juice as well as Vitis quinquangularis Vinegar and Mulberry Vinegar by HPLC

为向毛葡萄和桑椹深加工产品提供品控支持,开发了同时测定毛葡萄汁和桑椹汁及毛葡萄醋和桑椹醋中18种酚类物质的高效液相色谱方法,并建立了酚类物质的外标法定量。使用Atlantis^(■)T3色谱柱(4.6 mm×250 mm×5μm),以含1%乙酸的甲醇-乙腈(4∶6)为流动相A及1%乙酸-水溶液为流动相B,柱温30℃,流速1 mL/min,进样量5μL。通过洗脱梯度优化,实现了待测酚类物质的有效分离。方法学验证表明,18种酚类物质的标准曲线线性关系均≥0.9936,平均加标回收率在88.00%~107.46%之间,对应的RSD≤4.38%。结果表明,该方法操作简单,准确度高,可应用于工厂的毛葡萄汁和桑椹汁及毛葡萄醋和桑椹醋中酚类物质的定量品控。

In order to provide quality control support for the deep-processed products of Vitis quinquangularis and mulberry,a high performance liquid chromatography method for simultaneous determination of 18 phenolics in Vitis quinquangularis juice and mulberry juice as well as Vitis quinquangularis vinegar and mulberry vinegar is established,and an external standard method is established for the quantification of phenolics.Using Atlantis^(■)T3 chromatographic column(4.6 mm×250 mm×5μm),with methanol-acetonitrile(4∶6)containing 1%acetic acid as the mobile phase A and 1%acetic acid aqueous solution as the mobile phase B,column temperature is 30℃,flow rate is 1 mL/min,and sample volume is 5μL.Through the optimization of elution gradient,the effective separation of phenolics to be tested is realized.Methodological validation shows that the linear relationships of the standard curves of 18 phenolics are all≥0.9936,the average spiked recovery rate is between 88.00%and 107.46%,and the corresponding RSD is≤4.38%.The results show that this method is simple to operate and has high accuracy,indicating that it can be applied to the quantitative quality control of phenolics in Vitis quinquangularis juice and mulberry juice as well as Vitis quinquangularis vinegar and mulberry vinegar in factories.