胃癌组织中KLF6和PTTG1的表达与胃癌淋巴结转移及预后的关系

Relationship between the expression of KLF6 and PTTG1 and lymph node metastasis and prognosis in gastric cancer

目的:探讨Kruppel样因子6(KLF6)、垂体肿瘤转化基因1(PTTG1)在胃癌组织中的表达,并分析两者与胃癌淋巴结转移及预后的关系。方法:选取2018年4月—2019年10月诊治的80例胃癌患者,收集患者术中切除的癌组织及癌旁正常组织。采用免疫组织化学法对KLF6、PTTG1的表达进行检测;Spearman法分析胃癌组织中KLF6和PTTG1表达的相关性;Logistic回归分析影响淋巴结转移的因素;Kaplan-meier法分析胃癌组织中KLF6和PTTG1表达与患者预后的关系;Cox回归分析患者预后的影响因素。结果:与癌旁正常组织相比,胃癌组织中KLF6低表达率、PTTG1高表达率明显升高(P<0.05);胃癌组织中KLF6、PTTG1表达呈负相关(r=-0.502,P<0.05);胃癌淋巴结转移患者KLF6低表达、PTTG1高表达比例明显高于无淋巴结转移患者(P<0.05);KLF6是淋巴结转移的保护因素(P<0.05),PTTG1是淋巴结转移的危险因素(P<0.05)。KLF6高表达组生存率显著高于低表达组(χ^(2)=8.557,P<0.05),PTTG1低表达组生存率显著高于高表达组(χ^(2)=5.637,P<0.05);KLF6是患者预后的保护因素(P<0.05),淋巴结转移、PTTG1是患者预后的危险因素(P<0.05)。结论:胃癌组织中KLF6低表达、PTTG1高表达,均与胃癌淋巴结转移及预后有关。

Objective:To investigate the expression of Kruppel like factor 6(KLF6)and pituitary tumor transforming gene 1(PTTG1)in gastric cancer,and analyze their relationship with lymph node metastasis and prognosis of gastric cancer.Methods:Eighty patients with gastric cancer diagnosed and treated from April 2018 to October 2019 were selected,and the patients'intraoperative resected cancerous tissues and normal tissues adjacent to the cancer were collected.The expression of KLF6 and PTTG1 was detected by immunohistochemistry;the correlation between KLF6 and PTTG1 expression in gastric cancer was analyzed by Spearman method;Logistic regression was performed to analyze the factors affecting lymph node metastasis of gastric cancer;Kaplan-Meier method was used to analyze the relationship between the expression of KLF6 and PTTG1 and the prognosis of patients;COX regression was used to analyze the prognostic factors in patients.Results:Compared with normal adjacent tissues,the low expression rate of KLF6 and high expression rate of PTTG1 in gastric cancer tissues were obviously higher(P<0.05);the expression of KLF6 and PTTG1 in gastric cancer was negatively correlated(r=-0.502,P<0.05);the proportions of KLF6 low expression and PTTG1 high expression in patients with lymph node metastasis of gastric cancer were obviously higher than those in patients without lymph node metastasis(P<0.05);KLF6 was a protective factor for lymph node metastasis in patients(P<0.05),and PTTG1 was a risk factor for lymph node metastasis(P<0.05).The survival rate of patients with high KLF6 expression was obviously higher than that of patients with low KLF6 expression(χ^(2)=8.557,P<0.05),and the survival rate of patients with low PTTG1 expression was obviously higher than that of patients with high PTTG1 expression(χ^(2)=5.637,P<0.05);KLF6 was a protective factor for the prognosis of patients(P<0.05),while lymph node metastasis and PTTG1 were risk factors for the prognosis of patients(P<0.05).Conclusion:KLF6 is low expression and PTTG1 is high expression in gastric cancer,both are related to lymph node metastasis and prognosis of gastric cancer.