鸡肉加工及零售环节中分离的沙门氏菌血清型、毒力基因及耐药性检测

Detection of Serotype,Virulence Gene and Drug Resistance of Salmonella in Chicken Processing and Retail Links

为了研究河北省承德地区鸡肉加工及零售环节中沙门氏菌致病性、血清型分布、毒力基因及耐药性等生物学特性,对2021-2022年采集自河北省承德地区鸡屠宰场、加工厂及零售样品257份进行沙门氏菌分离鉴定,采用人工感染小鼠试验、玻板凝集试验、PCR方法及K-B药敏纸片法检测分离菌株的致病性、血清型、毒力基因及耐药性情况。结果显示,从257份样品中分离到70株致病性沙门氏菌,70株沙门氏菌中有12种血清型,其中以肠炎沙门氏菌(24.3%)、鼠伤寒沙门氏菌(21.4%)、都柏林沙门氏菌(17.1%)为流行优势血清型;毒力质粒基因spvA、spvB、spvC、spvR检出率在12.9%~88.6%之间,毒力岛基因SPI-1 SPI-2、SPI-3、SPI-5检出率在27.0%~90.0%之间;对阿莫西林、氨苄西林、磺胺间氧嘧啶等7种药物耐药率在51.4%以上,对其他药物的耐药率在5.7%~40%之间,以耐10(15.0%)、9(20.0%)、8(17.1%)种药物为主;耐药基因Sul1、Sul2、Sul3、TetA、TetM、TetR、aac(3)-Ⅰv、aac(6′)-Ⅰb检出率在45.7%~97.1%之间,其他耐药基因检出率在8.6%~17.1%之间,耐药表型与耐药基因之间(除酰胺醇类、多黏菌素类)基本呈正相关。结果表明,从承德地区鸡肉加工及零售环节中分离的70株致病性沙门氏菌具有多种血清型、携带多种毒力基因、耐药性严重,且携带多种耐药基因,耐药表型与耐药基因之间存在相关性。

To study the pathogenicity,serotype distribution,virulence gene and drug resistance of Salmonella in chicken processing and retail links in Chengde,Hebei province,in this experiment,257 samples from chicken slaughterhouses,processing plants and retail stores in Chengde,Hebei province were collected from 2021-2022 for isolation and identification of Salmonella.The pathogenicity,serotype,virulence gene and drug resistance of the isolated strains were detected by artificial infection mouse test,glass plate agglutination test,PCR method and K-B drug sensitive paper method.The results showed that 70 pathogenic Salmonella strains were isolated from 257 samples;There are 12 serotypes in 70 strains of Salmonella,among which Salmonella enteritidis(24.3%),Salmonella typhimurium(21.4%)and Salmonella dublin(17.1%)are the dominant serotypes;The detection rates of virulence plasmid genes(spvA,spvB,spvC,spvR)were between 12.9%and 88.6%,and the detection rates of virulence island genes(SPI-1,SPI-2,SPI-3,SPI-5)were between 27.0%and 90.0%;The drug resistance rate to 7 drugs,such as amoxicillin,ampicillin and sulfamethoxypyrimidine,was more than 51.4%,and the drug resistance rate to other drugs was between 5.7%and 40%,with 10(15.0%),9(20.0%)and 8(17.1%)drugs being the main resistance;The detection rate of drug resistance genes Sul1,Sul2,Sul3,TetA,TetM,TetR,aac(3)-Ⅰv and aac(6′)-Ⅰb was 45.7%-97.1%,and the detection rate of other drug resistance genes was between 8.6%and 17.1%.There was basically positive correlation between drug resistance phenotype and drug resistance genes(except amidohols and polymyxin).The results showed that 70 strains of pathogenic Salmonella isolated from chicken processing and retail links in Chengde area had multiple serotypes,carried multiple virulence genes,serious drug resistance,and carried multiple drug resistance genes.