黄牛源Fh/Fg杂合型片形吸虫的鉴定

Identification of Fh/Fg Hybrid Type of Fasciola Species from Yellow Cattle

为弄清黄牛感染的片形吸虫种类,首先对来自河南济源市一肉牛屠宰场黄牛肝脏上的片形吸虫进行形态学观察,然后基于核糖体ITS1、ITS2区域和线粒体nad1、cox1基因序列进行分子鉴定。ITS1和ITS2序列分析结果显示,济源黄牛源片形吸虫分离株JY003和JY004为Fh/Fg杂合型片形吸虫。基于nad1和cox1基因序列,JY003和JY004与大片形吸虫的相似性高于肝片形吸虫,其中nad1序列与大片形吸虫中国云南、日本和韩国分离株的相似性高达100%。在基于nad1和cox1基因的进化树上,分离株均与大片形吸虫参考株处于同一分支。结果表明,首次从河南黄牛体内鉴定出Fh/Fg杂合型片形吸虫,为动物和人的片形吸虫病防控提供重要的基础数据。

To reveal the species of Fasciola fluke in yellow cattle,adult parasites of Fasciola were isolated from the liver of a yellow cattle in a beef cattle slaughterhouse in Jiyuan of Henan province and observed morphologically.Then the parasites were further identified by sequence analysis based on the ribosomal ITS1 and ITS2 regions and mitochondrial nad1 and cox1 genes.Sequence analysis based on ITS1 and ITS2 regions showed that the isolates JY003 and JY004 from the affected yellow cattle in this study were the Fh/Fg hybrid type of Fasciola.The nad1 and cox1 sequences of JY003 and JY004 were more similar to that of F.gigantica than F.hepatica,especieally the nad1 sequences of the two isolates were 100%identical to that of the F.gigantica isolates from Yunnan of China,Japan and Korea.In the phylogenetic trees based on the nad1 and cox1 genes,the two isolates in this study were clustered into the same branch with the reference strains of F.gigantica.This is the first report of the Fh/Fg hybrid type of Fasciola infection in a yellow cattle from Henan province,providing important basic data for the prevention and control of fasciolosis in animals and humans.