基于InDel标记的杏鲍菇遗传多样性评价

Diversity evaluation of Pleurotus eryngii strains based on InDel markers

插入/缺失(insertion-deletion,InDel)标记作为快速鉴定品种间遗传多样性的方法之一,因具有对检测设备要求低、简单易行、成本低,且共显性遗传、变异稳定、准确性高、重复性好等特点,在医学、动植物遗传学的群体遗传分析、基因定位及遗传图谱构建等领域被广泛应用。为评价我国杏鲍菇菌株的遗传多样性,加快杏鲍菇遗传育种工作进程,通过比对杏鲍菇已公布的基因组组装数据,开发30~200 bp InDel标记便于常规电泳检测,随机选取合成36对引物对收集自国家食用菌标准菌株库、国内企业和市场的65份杏鲍菇菌株进行验证,筛选出25对有效引物,进行遗传多样性分析并构建UPGMA聚类树。结果表明,65份供试菌株遗传多样性丰富,遗传相似系数最低为0.362,在相似系数0.616时可分为5个类群。

Insertion deletion(InDel)markers,as one of the methods for quickly identifying genetic diversity between varieties,are widely used in fields such as medicine,animal and plant genetics,population genetic analysis,gene mapping,and genetic map construction due to their low requirements for detection equipment,simplicity,low cost,co dominant inheritance,stable variation,high accuracy,and good repeatability.In order to evaluate the genetic diversity of Pleurotus eryngii strains in China and accelerate the genetic breeding process,30~200 bp InDel markers were developed by comparing the published genome assembly data of Pleurotus eryngii for routine electrophoresis detection.36 pairs of primers were randomly selected and synthesized to validate 65 strains collected from the China Center for Mushroom Spawn Standards and Control,domestic enterprises,and markets.25 pairs of effective primers were screened and applied in genetic diversity analysis and UPGMA clustering tree construction.The results showed that the 65 strains were rich in genetic diversity,with the lowest genetic similarity coefficient of 0.362.These strains could be divided into 5 groups when the similarity coefficient was 0.616.