上皮性卵巢癌组织中CircITGB6、CircPBX3的表达变化及意义

Expression changes and clinical significance of CircITGB6 and CircPBX3 in epithelial ovarian cancer tissues

目的探讨环状RNA(Circ)ITGB6、CircPBX3在上皮性卵巢癌(EOC)组织中的表达变化及意义。方法选取接受卵巢癌根治术的80例EOC患者,应用实时荧光定量PCR检测癌及癌旁正常组织(距离癌组织≥3 cm)中CircITGB6、CircPBX3表达。Pearson相关分析EOC组织中circITGB6与CircPBX3表达的相关性。比较不同临床病理特征患者癌组织中CircITGB6、CircPBX3表达差异。Kaplan-Meier生存分析(Log-Rank检验)不同CircITGB6、CircPBX3表达EOC患者生存预后差异。Cox比例风险模型分析影响EOC患者预后的因素。结果EOC癌组织中CircITGB6、CircPBX3相对表达量分别为3.17±0.57、4.02±0.62,癌旁正常组织分别为1.06±0.24、1.23±0.32,癌组织中CircITGB6、CircPBX3相对表达量高于癌旁正常组织(t分别为30.515,35.766,P均<0.05)。EOC癌组织中CircITGB6与CircPBX3表达呈正相关(r=0.685,P<0.05)。肿瘤FIGO分期Ⅲ期、合并淋巴结转移的EOC患者癌组织中CircITGB6、CircPBX3相对表达量高于FIGO分期Ⅰ、Ⅱ期及无淋巴结转移癌组织(P均<0.05)。CircITGB6高表达和低表达者3年累积生存率分别为51.28%(20/39)、80.49%(33/41),CircITGB6高表达者3年累积生存率低于CircITGB6低表达者(χ^(2)=8.179,P<0.05)。CircPBX3高表达和低表达者3年累积生存率分别为55.26%(21/38)、76.19%(32/42),CircPBX3高表达者3年累积生存率低于CircPBX3低表达者(χ^(2)=5.763,P<0.05)。FIGO分期Ⅲ期、合并淋巴结转移、CircITGB6高表达、CircPBX3高表达是影响EOC患者不良生存预后的独立危险因素(P均<0.05)。结论EOC癌组织中CircITGB6、CircPBX3表达升高,二者表达与肿瘤FIGO分期、淋巴结转移有关,CircITGB6、CircPBX3升高是EOC患者不良生存预后的独立危险因素。

Objective To investigate the expression changes and significance of circular RNA(Circ)ITGB6 and Cir⁃cPBX3 in epithelial ovarian cancer(EOC)tissues.Methods Eighty patients with EOC who underwent radical operation for ovarian cancer were selected.Real-time fluorescence quantitative PCR was applied to detect the expression of CircIT⁃GB6 and CircPBX3 in cancer tissues and adjacent tissues(≥3 cm away from the cancer tissues).Pearson correlation analy⁃sis was applied to analyze the correlation between the expression of circITGB6 and CircPBX3 in EOC tissues.The expres⁃sion differences of CircITGB6 and CircPBX3 in cancer tissues with different clinical and pathological characteristics were compared.Kaplan-Meier survival analysis(Log Rank test)was used to analyze the difference in survival prognosis among EOC patients with different CircITGB6 and CircPBX3 expression levels.COX proportional risk model analysis was applied to analyze factors affecting the prognosis of EOC patients.Results The relative expression levels of CircITGB6 and CircP⁃BX3 in EOC cancer tissues were 3.17±0.57 and 4.02±0.62,respectively,which were higher than those in the normal ovarian tissues(1.06±0.24 and 1.23±0.32),with statistically significant difference(t=30.515,35.766,both P<0.05).There was a significant positive correlation between the expression of CircITGB6 and CircPBX3 in EOC cancer tissues(r=0.685,P<0.05).The relative expression levels of CircITGB6 and CircPBX3 in EOC cancer tissues with FIGO stage III and combined lymph node metastasis were significantly higher than those in FIGO stage I-II and no lymph node metastasis(both P<0.05).The overall 3-year survival rates of the high and low expression groups of CircITGB6 were 51.28%(20/39)and 80.49%(33/41),respectively.The overall 3-year survival rates of the high and low expression groups of CircPBX3 were 55.26%(21/38)and 76.19%(32/42),respectively.The 3-year cumulative survival rate of the high CircITGB6 expression group was significantly lower than that of low CircITGB6 expression group(Log Rankχ^(2)=8.179,P<0.05).The 3-year cumulative survival rate of the high CircPBX3 expression group was significantly lower than that of low CircPBX3 expression group(Log Rankχ^(2)=5.763,P<0.05).FIGO stage III,lymph node metastasis,high Cir⁃cITGB6 expression,and high CircPBX3 expression were independent risk factors for the poor survival prognosis of EOC pa⁃tients.Conclusions The expression of CircITGB6 and CircPBX3 are elevated in EOC cancer tissue,which are related to tumor FIGO staging and lymph node metastasis.Elevated CircITGB6 and CircPBX3 are independent risk factors for poor survival prognosis in EOC patients.