不同提取方式对北板蓝根多糖含量和抗氧化活性的影响

Effects of different extraction methods on polysaccharide content and antioxidant activity of northern Ban-Lan-Gen

考察不同提取方式对北板蓝根多糖含量及抗氧化活性的影响.采用常规回流提取法和超声波辅助回流法提取北板蓝根多糖,通过苯酚−硫酸分光光度法测定总多糖含量,采用二苯代苦味酰基自由基(2,2−diphenyl−1−picryhydrazyl,DPPH·)和2,2−联氮−二(3−乙基−苯并噻唑−6−磺酸)二铵盐自由基(ABTS^(+)·)清除实验评价体外抗氧化作用.结果表明,超声波辅助回流法提取的多糖得率为40.07%,显著高于常规回流提取法(13.42%).苯酚−硫酸法的精密度高,重复性、稳定性和加样回收率良好,可用于不同提取方法制备的北板蓝根总多糖含量研究.超声波辅助回流法提取的多糖含量比常规回流提取法提高22.3%,对DPPH自由基和ABTS+自由基的清除作用显著优于常规回流提取法.研究结果可为超声波技术在北板蓝根多糖提取中的应用提供参考.

The effects of different extraction methods on polysaccharide content and antioxidant activity of northern Ban-Lan-Gen were investigated.The polysaccharide of northern Ban-Lan-Gen was extracted by conventional reflux extraction method and ultrasonic assisted reflux extraction method.The total polysaccharide content was determined by phenol-sulfuric acid spectrophotometry.DPPH free radical and ABTS+free radical scavenging assays were used to evaluate their antioxidant activity in vitro.The results show that the yield of polysaccharide extracted by ultrasonic assisted reflux extraction method was 40.07%,which was significantly higher than that by conventional reflux extraction method(13.42%).The phenolsulfuric acid method has high precision,repeatability,stability and good recovery,which can be used to study the polysaccharide content of northern Ban-Lan-Gen prepared by different extraction methods.The polysaccharide content of ultrasonic assisted reflux extraction method was 22.3%higher than that of conventional reflux extraction method,and the scavenging effects of DPPH·and ABTS^(+)·was significantly better than that of conventional reflux extraction method.The results provide a reference for the application of ultrasonic technology in the extraction of polysaccharide of northern Ban-Lan-Gen.