贵州省一起灰花纹鹅膏中毒事件调查

Investigation of a mushroom poisoning incident caused by Amanita fuliginea in Guizhou Province

目的调查分析贵州省毕节市发生的一起灰花纹鹅膏中毒事件,为贵州省毒蘑菇中毒的诊断、调查、检测鉴定和防控提供数据支持。方法采用流行病学调查方法分析该起事件流行病学特征,采用分子生物学方法对可疑野生蘑菇进行鉴定,采用超高效液相色谱-串联质谱法进行毒素检测。结果该事件为食用自采野蘑菇共导致4人中毒,其中3人死亡。经流行病学调查和实验室检测,确定毒蘑菇为灰花纹鹅膏(Amanita fuliginea),α-鹅膏毒肽、β-鹅膏毒肽和二羟鬼笔毒肽毒素被检出。结论该起毒蘑菇中毒由村民误采误食灰花纹鹅膏引起,开展针对性的健康教育对毒蘑菇中毒的预防控制具有重要意义。

Objective To provide support and reference for the diagnosis,investigation,laboratory testing,and prevention of toxic mushroom poisoning in Guizhou,an Amanita fuliginea poisoning incident in Bijie,Guizhou Province was investigated.Methods An epidemiological investigation was conducted to analyze the epidemiological characteristics of the event.Molecular biology techniques were used to identify suspicious wild mushrooms.Ultra high-performance liquid chromatography tandem mass spectrometry was used to detect toxins.Results In this incident,three out of four people who ate wild mushrooms died.According to epidemiological and the experimental results,the mushroom was confirmed as Amanita fuliginea.α-amanitin,β-amanitin,and phallacidin were detected.Conclusion The poisoning incident was caused by the villagers mistakenly eating Amanita fuliginea.It is essential to conduct targeted health education for the prevention and control of toxic mushroom poisoning.