基于苯并噻唑的汞离子荧光探针的合成与应用研究

Synthesis and application of novel benzothiazole-based fluorescent probe with high selectivity for Hg^(2+)detection

以末端缩硫醛作为识别位点、苯并噻唑衍生物作为荧光团,设计并合成了一种具有特定反应的新型Hg^(2+)荧光探针(BDP)。它与Hg^(2+)结合时会促进硫解反应,生成具有强荧光的3-苯并噻唑-6-二乙氨基-2-羟基苯甲醛,从而达到荧光增强的效果。通过紫外-可见吸收光谱和荧光发射光谱研究了探针BDP对Hg^(2+)的识别效果,表明探针BDP可高选择性、高灵敏度地检测Hg^(2+),具有检出限低(14.9 nmol/L)、响应迅速等优良性能,已被成功应用于实际水样中Hg^(2+)的检测。

A novel reactive fluorescent probe(BDP)is designed and synthesized through using terminal thiol as recognition site and benzothiazole derivatives as fluorescent groups.As combined with Hg^(2+),BDP probe can undergo a thiolysis reaction to produce 3-benzothiazol-6-diethylamino-2-hydroxybenzaldehyde with strong fluorescence.The detection effect of BDP probe for Hg^(2+)is studied by means of UV-Vis absorption spectra and fluorescence emission spectra.The finding demonstrates that BDP probe shows rapid response,high selectivity and sensitivity to detect Hg^(2+)in solution under mild conditions.The detection limit is as high as 14.9 nmol·L^(-1).Additionally,the BDP probe has been successfully applied to the detection of Hg^(2+)in actual water samples.