大麦HvNRT2.3/HvNRT2.5基因对拟南芥生长发育及产量性状的影响

Effect of HvNRT2.3/HvNRT2.5 Originated from Barley on Development and Yield Related Traits in Transgenic Arabidopsis

高亲和硝酸盐转运蛋白(nitrate transporter,NRT)对于植物的氮代谢具有重要的作用。为研究大麦HvNRT2.3和HvNRT2.5基因的功能,本研究从大麦栽培品种‘Morex’克隆了大麦HvNRT2.3和HvNRT2.5基因,构建2个基因的超表达载体,转化拟南芥野生型(Col)。本研究测定了野生型和转基因株系的10个性状,结果显示超表达株系除株高没有显著变化外,转基因株系的莲座叶大小、荚果数目、长度、籽粒数目、千粒重、硝态氮含量和蛋白质含量等均显著增大或提高;成熟期单株干重分别增加45.63%和14.74%,单株籽粒产量分别提高31.37%和24.31%,收获指数较野生型没有显著变化。研究表明,过量表达大麦HvNRT2.3和HvNRT2.5基因能促进拟南芥生长,提高氮素的吸收能力和单株产量。

High-affinity nitrate transporter play an important role in nitrogen metabolism in plants.To study the functions of HvNRT2.3 and Hv NRT2.5 genes in barley,the study cloned both genes from barley cultivar 'Morex',and constructed the overexpression vector of them,and transformed Arabidopsis wild type(Col).In this study,10 traits of wild type and transgenic lines were determined.The results showed that except plant height,rosette leaf size,number of siliques per plant,silique number per plant,length of silique,thousand grain weight,nitrate content and grain protein were significantly increased in transgenic lines.At maturity,dry weight per plant increased by 45.63% and 14.74%,grain yield per plant increased by 31.37% and 24.31% in HvNRT2.3 and HvNRT2.5 transgenic lines,respectively,and harvest in dex had no significant change compared with wild type.The results showed that heterologous expression of HvNRT2.3/HvNRT2.5 genes alone could promote growing and increase yield,as well as improve absorbing nitrogen ability in Arabidopsis thaliana.