1型糖尿病患者的B细胞及B细胞亚群中程序性死亡受体配体1的表达

Expression of programmed death receptor ligand 1 in B cells and B cell subsets of patients with type 1 diabetes mellitus

目的探讨1型糖尿病(T1DM)患者循环B细胞中程序性死亡受体配体1(PD-L1)的表达情况。方法采集健康对照者(n=25)和T1DM患者(n=25)的外周血标本。流式细胞术检测B细胞及其表面PD-L1的表达。结果T1DM患者B细胞亚群频率与健康对照者比较,差异无统计学意义(P>0.05)。与健康对照者相比,T1DM患者CD19^(+)细胞、CD19^(+)CD27^(+)细胞和CD19^(+)CD27^(+)细胞上PD-L1的表达降低,差异有统计学意义(P<0.05)。健康对照者与T1DM患者的PD-L1在B10细胞、边缘区B细胞(MZB)和滤泡B细胞(FoB)上的表达比较,差异无统计学意义(P>0.05)。T1DM患者的PD-L1在过渡性T2-边缘区前体B细胞(T2-MZP)上的表达低于健康对照者,差异有统计学意义(P<0.05)。结论PD-L1在T1DM发病中可能起保护作用。PD-L1高表达的B细胞可能为自身免疫性糖尿病患者的治疗提供新策略。

Objective To investigate the expression of programmed death receptor ligand 1(PD-L1)in circulating B cells of patients with type 1 diabetes mellitus(T1DM).Methods Peripheral blood samples were collected from healthy controls(n=25)and T1DM patients(n=25).The expression of PD-L1 on B cells and their surfaces was detected by flow cytometry.Results There was no significant difference in the frequency of B cell subsets between the T1DM patients and healthy controls(P>0.05).Compared with healthy controls,the expression of PD-L1 on CD19^(+)cells,CD19^(+)CD27^(+)cells and CD19^(+)CD27^(+)cells in the T1DM patients was significantly decreased(P<0.05).There was no significant difference in the expression of PD-L1 in B10 cells,marginal zone B cells(MZB)and follicular B cells(FoB)between healthy controls and T1DM patients(P>0.05).The expression of PD-L1 on transitional 2-marginal zone precursor B cells(T2-MZP)cells in the T1DM patients was lower than that in the healthy controls(P<0.05).Conclusion The PD-L1 may play a protective role in the pathogenesis of T1DM.B cells with high expression of PD-L1 may provide a new strategy for the treatment of patients with autoimmune diabetes.