刺五加精制多糖对尼古丁诱导的学习记忆障碍小鼠的影响

Effects of acanthopanax refined polysaccharide in mice with nicotine-induced learning and memory impairment

目的观察刺五加精制多糖(ASPS)对尼古丁诱导的学习记忆障碍小鼠的影响。方法选取6周龄小鼠96只,雌雄各半,将其分为2批进行动物试验,即水迷宫试验和新物体识别试验。每批按小鼠体质量随机分为6组:空白组、模型组、阳性药物组、ASPS高剂量组、ASPS中剂量组、ASPS低剂量组。除空白组外,其余5组连续7 d每天皮下注射尼古丁0.5 mg/kg,制备尼古丁记忆障碍模型。第7天注射尼古丁24 h后,阳性药物组灌胃吡拉西坦800 mg/kg,ASPS高剂量组、ASPS中剂量组、ASPS低剂量组连续7 d分别灌胃ASPS 270、90、30 mg/kg。通过水迷宫试验和新物体识别试验分别检测小鼠的学习记忆能力。测试结束后,检测血清中超氧化物歧化酶(SOD)活性和海马组织中五羟色胺(5-HT)含量。结果新物体识别试验结果显示,ASPS高、中、低剂量组辨别指数高于模型组,差异有统计学意义(P<0.01或P<0.05)。水迷宫试验结果显示,与模型组比较,ASPS高、中剂量组小鼠空间搜索试验中找到平台的时间缩短,差异有统计学意义(P<0.05);定位航行试验中,ASPS高剂量组小鼠平台进入次数多于模型组,差异有统计学意义(P<0.05);ASPS高、中剂量组小鼠Ⅲ象限路程比例高于模型组,差异有统计学意义(P<0.05)。ASPS高、中、低剂量组SOD活性高于模型组,差异有统计学意义(P<0.01);小鼠海马组织含量测定显示,ASPS高、中剂量组小鼠5-HT含量高于模型组,差异有统计学意义(P<0.01或P<0.05)。结论ASPS能显著提高尼古丁戒断小鼠的学习记忆能力,减轻海马神经递质的损伤,调节体内氧化应激能力,其机制可能与提高机体抗氧化能力、调节海马神经递质水平有关。

Objective To observe the effect of acanthopanax refined polysaccharide(ASPS)on nicotine-induced learning and memory impairment in mice.Methods A total of 48 male mice and 48 females at 6 weeks of age were selected and were divided into two batches for animal experiments:the Morris and the new object recognition batch.Each batch was randomly divided into 6 groups according to body weight:blank control group,model group,drug positive group,high-dose ASPS group,medium-dose ASPS group,and low-dose ASPS group.Except for the blank group,the remaining 5 groups were injected subcutaneously with 0.5mg/kg of nicotine every day for 7 days to prepare a nicotine memory disorder model.After 24 hours of injection of nicotine at the 7 th day,the drug positive group was gavaged piracetam for 800 mg/kg,and the high-dose,medium-dose and low-dose ASPS groups were gavaged for 270,90 and 30 mg/kg of ASPS respectively for 7 days.The learning and memory ability of mice was detected by water maze test and new object recognition test,respectively.After the two tests,superoxide dismutase(SOD)activity in serum and 5-Hydroxytryptamine(5-HT)content in hippocampal tissue were detected.Results The results of new object recognition experiment showed that the discrimination indexes of the high-,medium-and low-dose ASPS groups were significantly higher than that of the model group(P<0.01 or P<0.05).The results of water maze experiment showed that the time to find the platform in the spatial search experiment was significantly shorter in the high-and medium-dose ASPS groups than that in the model group(P<0.05).In the positioning voyage test,the number of mouse platform entries in the high-dose ASPS group was significantly more than that in the model group(P<0.05);the proportions ofⅢquadrant routes in the high-and medium-dose ASPS groups were higher than those in the model group(P<0.05).The high-,medium-and low-dose ASPS groups were significantly higher than those in the model group(P<0.01);the determination of hippocampal tissue content in mice showed that the content of 5-HT in the high-and medium-dose ASPS groups was significantly higher than that in the model group(P<0.01 or P<0.05).Conclusion ASPS can significantly improve the learning and memory ability of nicotine-quitting mice,relieve the damage of hippocampal neurotransmitters,and regulate oxidative stress in vivo.The mechanism may be related to improving the body's antioxidant capacity and regulating hippocampal neurotransmitter levels.