摘要
目的观察刺五加精制多糖(ASPS)对尼古丁诱导的学习记忆障碍小鼠的影响。方法选取6周龄小鼠96只,雌雄各半,将其分为2批进行动物试验,即水迷宫试验和新物体识别试验。每批按小鼠体质量随机分为6组:空白组、模型组、阳性药物组、ASPS高剂量组、ASPS中剂量组、ASPS低剂量组。除空白组外,其余5组连续7 d每天皮下注射尼古丁0.5 mg/kg,制备尼古丁记忆障碍模型。第7天注射尼古丁24 h后,阳性药物组灌胃吡拉西坦800 mg/kg,ASPS高剂量组、ASPS中剂量组、ASPS低剂量组连续7 d分别灌胃ASPS 270、90、30 mg/kg。通过水迷宫试验和新物体识别试验分别检测小鼠的学习记忆能力。测试结束后,检测血清中超氧化物歧化酶(SOD)活性和海马组织中五羟色胺(5-HT)含量。结果新物体识别试验结果显示,ASPS高、中、低剂量组辨别指数高于模型组,差异有统计学意义(P<0.01或P<0.05)。水迷宫试验结果显示,与模型组比较,ASPS高、中剂量组小鼠空间搜索试验中找到平台的时间缩短,差异有统计学意义(P<0.05);定位航行试验中,ASPS高剂量组小鼠平台进入次数多于模型组,差异有统计学意义(P<0.05);ASPS高、中剂量组小鼠Ⅲ象限路程比例高于模型组,差异有统计学意义(P<0.05)。ASPS高、中、低剂量组SOD活性高于模型组,差异有统计学意义(P<0.01);小鼠海马组织含量测定显示,ASPS高、中剂量组小鼠5-HT含量高于模型组,差异有统计学意义(P<0.01或P<0.05)。结论ASPS能显著提高尼古丁戒断小鼠的学习记忆能力,减轻海马神经递质的损伤,调节体内氧化应激能力,其机制可能与提高机体抗氧化能力、调节海马神经递质水平有关。
Objective To observe the effect of acanthopanax refined polysaccharide(ASPS)on nicotine-induced learning and memory impairment in mice.Methods A total of 48 male mice and 48 females at 6 weeks of age were selected and were divided into two batches for animal experiments:the Morris and the new object recognition batch.Each batch was randomly divided into 6 groups according to body weight:blank control group,model group,drug positive group,high-dose ASPS group,medium-dose ASPS group,and low-dose ASPS group.Except for the blank group,the remaining 5 groups were injected subcutaneously with 0.5mg/kg of nicotine every day for 7 days to prepare a nicotine memory disorder model.After 24 hours of injection of nicotine at the 7 th day,the drug positive group was gavaged piracetam for 800 mg/kg,and the high-dose,medium-dose and low-dose ASPS groups were gavaged for 270,90 and 30 mg/kg of ASPS respectively for 7 days.The learning and memory ability of mice was detected by water maze test and new object recognition test,respectively.After the two tests,superoxide dismutase(SOD)activity in serum and 5-Hydroxytryptamine(5-HT)content in hippocampal tissue were detected.Results The results of new object recognition experiment showed that the discrimination indexes of the high-,medium-and low-dose ASPS groups were significantly higher than that of the model group(P<0.01 or P<0.05).The results of water maze experiment showed that the time to find the platform in the spatial search experiment was significantly shorter in the high-and medium-dose ASPS groups than that in the model group(P<0.05).In the positioning voyage test,the number of mouse platform entries in the high-dose ASPS group was significantly more than that in the model group(P<0.05);the proportions ofⅢquadrant routes in the high-and medium-dose ASPS groups were higher than those in the model group(P<0.05).The high-,medium-and low-dose ASPS groups were significantly higher than those in the model group(P<0.01);the determination of hippocampal tissue content in mice showed that the content of 5-HT in the high-and medium-dose ASPS groups was significantly higher than that in the model group(P<0.01 or P<0.05).Conclusion ASPS can significantly improve the learning and memory ability of nicotine-quitting mice,relieve the damage of hippocampal neurotransmitters,and regulate oxidative stress in vivo.The mechanism may be related to improving the body's antioxidant capacity and regulating hippocampal neurotransmitter levels.
作者
邹婷
刘友昊
高晓岩
邓代千
吴宜艳
ZOU Ting;LIU Youhao;GAO Xiaoyan;DENG Daiqian;WU Yiyan(Mudanjiang Medical University,Mudanjiang,Heilongjiang,157011;Red Flag Hospital Affiliated to Mudanjiang Medical College,Mudanjiang,Heilongjiang,157011)
出处
《实用临床医药杂志》
2023年第22期67-70,76,共5页
Journal of Clinical Medicine in Practice
关键词
刺五加精制多糖
学习记忆
抗氧化作用
神经递质
超氧化物歧化酶
五羟色胺
eleuthero refined polysaccharides
learning and memory
antioxidant effect
neurotransmitter
superoxide dismutase
5-Hydroxytryptamine