UC患儿肠道菌群及血清MUC1、SOCS-3的表达水平与病情程度的相关性

Correlation between the expression levels of intestinal flora,serum membrane-bound mucin 1,and suppressors of cytokine signaling 3 and the severity of the disease in children with ulcerative colitis

目的探讨溃疡性结肠炎(UC)患儿肠道菌群及血清上皮细胞膜结合黏液素1(MUC1)、细胞因子信号转导负调控因子-3(SOCS-3)的表达水平与病情程度的相关性。方法回顾性分析2018年1月至2022年10月榆林市第一医院收治的110例UC患儿的临床资料,根据溃疡性结肠炎严重程度的不同将患儿分为轻度组35例,中度组46例和重度组29例,另选取同期30例健康体检者作为对照组。检测所有受检者粪便样品中的肠道菌群分布情况以及血清MUC1、SOCS-3水平,并采用Pearson相关性分析法分析各指标与UC病情严重程度的相关性。结果血清MUC1水平比较,重度组>中度组>轻度组>对照组,SOCS-3水平比较,重度组<中度组<轻度组<对照组,差异均有统计学意义(P<0.05);重度组与中度组患儿的双歧杆菌、乳杆菌、真杆菌明显少于轻度组与对照组,而肠杆菌、肠球菌、小梭菌明显多于轻度组与对照组,差异均有统计学意义(P<0.05),但重度组与中度组、轻度组与对照组的上述各项指标比较差异均无统计学意义(P>0.05)。经Pearson相关性分析结果显示,MUC1、肠杆菌、肠球菌、小梭菌与UC患儿病情严重程度呈正相关(r=0.639、0.221、0.420、0.316,P<0.05),SOCS-3、双歧杆菌、乳杆菌、真杆菌与UC患儿病情严重程度呈负相关(r=-0.481、-0.257、-0.252、-0.132,P<0.05)。结论UC患儿病情越严重,肠道致病菌越多,益生菌越少,且血清MUC1与UC患儿病情严重程度呈正相关,SOCS-3与UC患儿病情严重程度呈负相关。

Objective To investigate the correlation between the expression of intestinal flora,serum epithelial cell membrane-bound mucin 1(MUC1),and suppressors of cytokine signaling 3(SOCS-3)expression level and the severity of disease in children with ulcerative colitis(UC).Methods The clinical data of 110 children with UC admitted to the First Hospital of Yulin from January 2018 to October 2022 were retrospectively analyzed.According to the severity of ulcerative colitis,the children were divided into a mild group(35 cases),a moderate group(46 cases),and a severe group(29 cases).Another 30 healthy subjects were selected as the control group during the same period.The intestinal flora distribution in fecal samples and serum MUC1 and SOCS-3 levels of all subjects were detected,and the correlation between each index and the severity of UC was analyzed by Pearson correlation analysis.Results The serum MUC1 level were compared:severe group>moderate group>mild group>control group,the and the level of SOCS-3 were compared,severe group<moderate group<mild group<control group;the differences were statistically significant(P<0.05).The Bifidobacteria,Lactobacilli,and Eubacillus in the severe group and moderate groups were significantly less than those in the mild and control groups,while the Enterobacterium,Enterococcus and Clostridium were significantly more than those in the mild group and the control group,with statistically significant differences(P<0.05);there was no statistically significant difference in the above indicators between the severe and moderate groups,and aslo between the mild and control groups(P>0.05).The results of Pearson correlation analysis showed that MUC1,Enterobacter,Enterococcus,and Clostridium were positively correlated with the severity of UC(r=0.639,0.221,0.420,0.316,P<0.05),and SOCS-3,Bifidobacterium,Lactobacillus,and Eubacillus were negatively correlated with the severity of UC in children(r=-0.481,-0.257,-0.252,-0.132,P<0.05).Conclusion The more severe the disease of UC in children,the more intestinal pathogens and the less probiotics.Serum MUC1 was positively correlated with the severity of UC in children's disease,and SOCS-3 was negatively correlated with the severity of UC.