干扰素体外释放酶联免疫检验技术在结核病临床诊断中的应用

Application of in Vitro Release of Interferon Enzyme Linked Immunoassay in the Clinical Diagnosis of Tuberculosis

目的评价结核病临床诊断中,采用干扰素体外释放酶联免疫检验技术的有效性。方法选取2022年2月—2023年2月聊城市第四人民医院检验科98例疑似结核病患者,均提供结核菌素皮肤过敏试验、干扰素体外释放酶联免疫检验技术诊断,将痰培养结果作为诊断金标准,对比两种方法的诊断效果。结果痰培养显示,阳性检出率40.82%,结核菌素皮肤过敏试验检测阳性检出率48.98%,干扰素体外释放酶联免疫检验技术检测阳性检出率41.84%。干扰素体外释放酶联免疫检验技术的特异度与准确性均高于结核菌素皮肤过敏试验,差异有统计学意义(P<0.05);两种检验方法的敏感度比较,差异无统计学意义(P>0.05)。结论结核病临床诊断中,采用干扰素体外释放酶联免疫检验技术具有较高的诊断准确率,诊断效能较好。

Objective To evaluate the effectiveness of in vitro interferon release enzyme linked immunoassay in the clinical diagnosis of tuberculosis.Methods From February 2022 to February 2023,98 suspected tuberculosis patients from the laboratory department of Liaocheng Fourth People's Hospital were selected,all of whom provided tuberculin skin allergy test and interferon in vitro release enzyme-linked immunosorbent assay for diagnosis.The sputum culture results were used as the diagnostic gold standard,compared the diagnostic effects of the two methods.Results Sputum culture showed positive detection rate of 40.82%,positive detection rate of 48.98%in the tuberculin skin allergy test,and positive detection rate of 41.84%in the interferon in vitro release enzyme-linked immunosorbent assay.The specificity and accuracy of interferon in vitro release enzyme-linked immunosorbent assay technology were higher than those in tuberculin skin allergy test,and the difference was statistically significant(P<0.05).There was no statisti-cally significant difference in sensitivity between the two test methods(P>0.05).Conclusion In the clinical diagnosis of tuberculosis,the technique of interferon in vitro release enzyme linked immunoassay has high diagnostic accuracy,good diagnostic efficiency.