纳米氧化铁抑制鸡巨噬细胞沙门氏菌感染的作用研究

Inhibitory Effect of Iron Oxide Nanozymes on Salmonella Infection in Chicken Macrophages

为研究纳米氧化铁对鸡巨噬细胞沙门氏菌感染的抑制作用,试验选择鸡巨噬细胞(HD11)进行沙门氏菌攻毒,并加入纳米氧化铁处理,对细胞形态、活力、抑菌率、活性氧水平及自噬蛋白表达等指标进行检测。结果显示:与对照组相比,在20~200μg/mL浓度梯度内,纳米氧化铁对HD11细胞活力无显著影响(P>0.05),但对HD11细胞内的沙门氏菌均有显著抑制作用(P<0.05),抑菌率为28.7%~66.2%;沙门氏菌和纳米氧化铁在单独和共同处理的情况下,均显著提高HD11细胞的活性氧水平(P<0.05);沙门氏菌和纳米氧化铁共同处理,显著提高细胞自噬蛋白的表达(P<0.05)。研究表明,纳米氧化铁抑制HD11细胞内沙门氏菌的感染,从而为沙门氏菌减抗防治研制提供新思路。

In order to investigate the inhibitory effect of iron oxide nanozymes on Salmonella infection in chicken macro-phages,chicken macrophages(HD11)were used for Salmonella challenge and iron oxide nanozymes was added for treatment.Af-ter treatment,indicators such as cell morphology,viability,antibacterial rate,reactive oxygen species(ROS)level,and autophagy protein expression were detected.The results showed that compared with the control group,iron oxide nanozymes had no signifi-cant effect on viability within the concentration gradient between 20 and 200μg/mL(P>0.05).However,iron oxide nanozymes had a significant inhibitory effect on Salmonella in HD11 cells within the concentration gradient(P<0.05),and the antibacterial rate was 28.7%to 66.2%.Compared with the control group,Salmonella and iron oxide nanozymes significantly increased the ROS level of HD11 cells when treated alone or together(P<0.05).Compared with the control group,the combination of Salmonella and iron oxide nanozymes could significantly increase the expression of autophagic proteins in HD11 cells(P<0.05).The results sug-gested that iron oxide nanozymes had a protective effect on HD11 cells infected with Salmonella,and provided new ideas for the development of reduced antibiotics prevention and treatment of Salmonella.