含钼的硅酸盐生物陶瓷释放的化学离子对肌腱-骨相关的多细胞调控

Regulation of Tendon-bone Related Multiple Cells by Chemical Ions Released from Mo-containing Silicate Bioceramics

研究生物材料所产生的化学信号与细胞行为之间的作用关系,对于高性能组织工程材料的设计与制备具有重要的指导意义.本工作中,采用化学共沉淀法成功地合成了含钼(Mo)的硅酸盐(MS)生物活性陶瓷粉体,并探究其降解产生的活性离子化学信号对肌腱-骨相关细胞(肌腱干/祖细胞(TSPCs)和骨髓间充质干细胞(BMSCs))的调控作用.所合成的MS生物陶瓷颗粒大小均一,其降解后的浸提液中含有Mo、钙(Ca)、硅(Si)三种生物活性离子.特定浓度范围的MS浸提液可以同时促进TSPCs和BMSCs的增殖和迁移活性.更重要的是,MS浸提液可以同时刺激TSPCs和BMSCs的特异性分化,说明MS生物陶瓷释放的化学离子所构建的微环境,能够同时协调TSPCs和BMSCs的细胞行为.因此,MS生物陶瓷有望作为一种有效的“生物活性因子”用于肌腱-骨损伤修复.

Understanding the relationship between the chemical signals generated by biological materials and cellular behaviors has great significance for the design and preparation of high-performance tissue engineering biomaterials.In the past several decades,silicate bioceramics have been widely used in tissue engineering.Bioactive ions released from silicate bioceramics can act as chemical signals to regulate cellular behaviors and promote the tissue regeneration.Moreover,by regulating the components of silicate bioceramics,silicate bioceramics can generate specific chemical signals to regulate cellular behaviors of multiple cells.Here,by introducing molybdenum(Mo)element into silicate bioceramics,we have successfully developed Mo-containing silicate(MS)bioceramics which are able to regulate cellular behaviors of tendon stem/progenitor cells(TSPCs)and bone marrow mesenchymal stem cells(BMSCs)simultaneously.Using ammonium molybdate as a source of Mo element,MS bioceramics were prepared by chemical coprecipitation method.The synthesized MS bioceramics were mostly below 10μm in size and had uniform distribution of Mo elements.Moreover,MS bioceramics were composed of high-purity CaMoO_(4) and CaSiO_(3).To explore the effect of chemical signals generated from MS bioceramics on TSPCs and BMSCs,we prepared MS extracts for cell culture.MS bioceramics supported the survival of TSPCs and BMSCs and maintained a better cellular state during the culture period of 5 d.Due to the released Ca,Si and Mo ions from MS bioceramics,TSPCs and BMSCs cultured with MS extracts exhibited excellent proliferation and migration activities.Interestingly,after cultured with MS extracts in the appropriate concentration,the expression of osteogenic genes and protein of BMSCs and the expression of tenogenic genes and protein of TSPCs were significantly enhanced,suggesting that chemical signals generated by MS bioceramics simultaneously promoted the specific differentiation of TSPCs and BMSCs.Such MS bioceramics are believed to be an effective“bioactive factor”for repairing injury at the tendon-bone interfaces.