子宫内膜癌组织LncRNA OGFRP1、TDRG1表达变化及其与PI3K/AKT信号通路和预后的关系

Expression changes of LncRNA OGFRP1 and TDRG1 in endometrial carcinoma and their relationships with PI3K/AKT signaling pathway and prognosis

目的探讨子宫内膜癌(EC)组织长链非编码核糖核酸(LncRNA)阿片类生长因子受体假基因1(OGFRP1)、睾丸发育相关基因1(TDRG1)表达变化及其与磷脂酰肌醇3激酶/蛋白激酶B(PI3K/AKT)信号通路和预后的关系。方法选取EC患者90例,采用RT-qPCR法检测EC组织与癌旁组织中LncRNA OGFRP1、TDRG1及PI3K、AKT mRNA。采用Pearson相关性分析EC组织中LncRNA OGFRP1、TDRG1与PI3K、AKT mRNA表达的相关性。根据EC组织中LncRNA OGFRP1、TDRG1的表达均值将患者分为LncRNA OGFRP1、TDRG1高表达者及低表达者,K-M法绘制EC组织中不同LncRNA OGFRP1、TDRG1表达患者的生存曲线,Log-Rank检验分析EC组织LncRNA OGFRP1、TDRG1表达与患者预后的关系;COX回归分析EC患者预后的影响因素。结果EC组织中LncRNA OGFRP1、TDRG1及PI3K、AKT mRNA表达均高于癌旁组织(P均<0.05)。Pearson相关性分析显示,EC组织中LncRNA OGFRP1表达与PI3K、AKT mRNA表达呈正相关(r分别为0.584、0.571,P均<0.01),LncRNA TDRG1表达与PI3K、AKT mRNA表达呈正相关(r分别为0.593、0.584,P均<0.01)。LncRNA OGFRP1、TDRG1高表达的EC患者3年总生存率低于LncRNA OGFRP1、TDRG1低表达患者(P均<0.05)。Cox回归分析显示,FIGO分期Ⅲ期、淋巴结转移、LncRNA OGFRP1≥1.19、LncRNA TDRG1≥1.35为影响EC患者预后的独立危险因素(P均<0.05)。结论EC组织中LncRNA OGFRP1、TDRG1表达升高,LncRNA OGFRP1、TDRG1表达与PI3K/AKT信号通路相关分子表达存在相关性,且为EC患者预后不良的独立危险因素。

Objective To investigate the changes in the expression levels of long non-coding RNA(LncRNA)opi⁃oid growth factor receptor pseudogene 1(OGFRP1)and testicular development related gene 1(TDRG1)in endometrial carcinoma(EC)tissues,and their relationships with phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)signal⁃ing pathway and prognosis.Methods Ninety patients with EC were selected.The expression levels of LncRNA OG⁃FRP1,TDRG1 and PI3K,AKT mRNA in EC tissues and paraneoplastic tissues of the patients were detected by RT-qP⁃CR.Pearson correlation was used to analyze the correlations between LncRNA OGFRP1,TDRG1 and PI3K,AKT mRNA expression in EC tissues.Based on the mean values of LncRNA OGFRP1 and LncRNA TDRG1 expression in EC tissues,patients were classified into those with high expression of LncRNA OGFRP1 and LncRNA TDRG1 and those with low ex⁃pression,and the survival curves of the patients with different expression of LncRNA OGFRP1 and LncRNA TDRG1 in EC tissues were plotted using the K-M method.The relationships between LncRNA OGFRP1 and LncRNA TDRG1 expression and patients'prognosis in EC tissues were analyzed by Log-Rank test.COX regression was used to analyze the factors af⁃fecting the prognosis of EC patients.Results LncRNA OGFRP1,LncRNA TDRG1,and PI3K and AKT mRNA expres⁃sion were higher in the EC tissues than in the paraneoplastic tissues(all P<0.05).Pearson correlation analysis showed that LncRNA OGFRP1 expression was positively correlated with PI3K mRNA and AKT mRNA expression levels in EC tis⁃sues(r=0.584,0.571,respectively;all P<0.01),and LncRNA TDRG1 expression was positively correlated with PI3K mRNA and AKT mRNA expression levels(r=0.593,0.584,respectively;all P<0.01).The 3-year overall survival rates of EC patients with high expression levels of LncRNA OGFRP1 and TDRG1 were lower than those of patients with low ex⁃pression levels of LncRNA OGFRP1 and TDRG1(all P<0.05).Cox regression analysis showed that federation internation⁃al of gynecology and obstetrics stage III,lymph node metastasis,LncRNA OGFRP1≥1.19,and LncRNA TDRG1≥1.35 were independent risk factors affecting the prognosis of patients with EC(all P<0.05).Conclusion LncRNA OGFRP1 and LncRNA TDRG1 expression levels increased in EC tissues,there were correlations between the expression levels of LncRNA OGFRP1 and LncRNA TDRG1 and expression levels of molecules related to the PI3K/AKT signaling pathway,and they were independent risk factors for poor prognosis of EC patients.