补骨益髓饮治疗骨质疏松大鼠的作用机理研究

Study on the Mechanism of Bugu Yisui Decoction in the Treatment of Osteoporosis Rats

目的研究补骨益髓饮对骨质疏松大鼠治疗的作用机理。方法取60只大鼠建立骨质疏松性骨折大鼠模型,采用随机数字表法分为试药组、阳性对照组、模型组,各组30只。试药组灌胃给予补骨益髓饮,阳性对照组灌胃给予睾酮,模型组给予等体积蒸馏水,另选取20只作为空白组。比较各组大鼠左侧胫骨骨折部位骨密度值,观察骨组织形态学变化。并检测大鼠血清中前列腺素E2、前列环素变化、胫骨组织中基因和蛋白表达。结果模型组大鼠胫骨组织状态较空白组变差,组织病理改变严重;与空白组比较,模型组大鼠血清中PGE2与PGI2均升高(P<0.05);与模型组比较,试药组大鼠血清中PGE2和PGI2含量均降低(P<0.05)。阳性对照组与试药组大鼠血清PGE2和PGI2水平比较,差异无统计学意义(P>0.05);与空白组比较,模型组大鼠胫骨组织BMP2、TIMP1蛋白表达降低,试药组大鼠胫骨组织TIMP1蛋白表达与模型组比较呈上升趋势(P<0.05);与阳性对照组比较,蛋白表达增高(P<0.05);试药组大鼠胫骨组织BMP2蛋白表达相对于模型组增加(P<0.05),与阳性对照组比较,差异无统计学意义(P>0.05);与空白组比较,模型组大鼠胫骨组织BMP2、TIMP1基因表达下降,差异有统计学意义(P<0.05);试药组大鼠胫骨组织BMP2和TIMP1基因表达高于模型组和阳性对照组(P<0.05),阳性对照组大鼠胫骨组织BMP2和TIMP1基因表达均高于模型组(P<0.05)。结论补骨益髓饮对骨质疏松大鼠模型有一定的治疗作用,相关机制可能与其抑制大鼠血清PGE2/PGI2水平,促进BMP2/TIMP1基因及蛋白表达,改善骨质量有关。

Objective To study the mechanism of Bugu Yisui decoction in the treatment of osteoporosis rats.Methods A total of 60 rats were used to establish a rat model of osteoporotic fracture.They were divided into test group,positive control group and model group by random number table method,with 30 rats in each group.The test group was given Bugu Yisui decoction by gavage,the positive control group was given testosterone by gavage,and the model group was given equal volume of distilled water.Another 20 rats were selected as the blank group.The bone mineral density of the left tibia fracture site of each group was compared,and the morphological changes of bone tissue were observed.The changes of prostaglandin E2 and prostacyclin in serum,gene and protein expression in tibia tissue were detected.Results The tibial tissue status of the model group was worse than that of the blank group,and the histopathological changes were serious.Compared with the blank group,PGE2 and PGI2 in the serum of the model group were increased(P<0.05).Compared with the model group,the contents of serum PGE2 and PGI2 of rats in the test group were decreased(P<0.05).There was no significant difference in serum PGE2 and PGI2 levels between the positive control group and the test group(P>0.05).Compared with the blank group,the expression of BMP2 and TIMP1 protein in the tibial tissue of the model group decreased,and the expression of TIMP1 protein in the tibial tissue of the test group increased compared with the model group(P<0.05).Compared with the positive control group,the protein expression was increased(P<0.05).Compared with the model group,the expression of BMP2 protein in the tibia tissue of the rats in the test group was increased(P<0.05),and there was no significant difference compared with the positive control group(P>0.05).Compared with the blank group,the expression of BMP2 and TIMP1 genes in the tibia tissue of the model group decreased,and the difference was statistically significant(P<0.05).The gene expression of BMP2 and TIMP1 in tibia tissue of rats in the test drug group was higher than that in the model group and the positive control group(P<0.05),and the gene expression of BMP2 and TIMP1 in tibia tissue of rats in the positive control group was higher than that in the model group(P<0.05).Conclusion Bugu Yisui decoction has a certain therapeutic effect on osteoporosis rat model,and the related mechanism may be related to its inhibition of serum PGE2/PGI2 level,promotion of BMP2/TIMP1 gene and protein expression,and improvement of bone quality.