DCST1-AS1在非小细胞肺癌中的表达及其生物学作用

Expression and biological role of DCST1-AS1 in non-small cell lung cancer

目的检测DCST1-AS1在非小细胞肺癌中的表达,并探讨其对癌细胞恶性生物学行为的影响。方法收集65例非小细胞肺癌患者的癌组织及其癌旁组织,体外培养正常人支气管上皮细胞16HBE和非小细胞肺癌细胞系(A549、H1299、H1650和HCC827)。RT-qPCR法检测组织和细胞中DCST1-AS1、miR-29b表达水平,并分析DCST1-AS1表达与非小细胞肺癌患者临床特征的相关性。将A549细胞分为对照组、si-NC组、si-DCST1-AS1组、si-DCST1-AS1+anti-NC组和si-DCST1-AS1+anti-miR-29b组。采用CCK-8法、克隆形成实验检测细胞增殖;Transwell检测细胞侵袭及迁移;Western blot检测E-cadherin、Vimentin、N-cadherin表达。结果非小细胞肺癌组织和细胞中DCST1-AS1的表达均升高(P<0.05),miR-29b的表达均降低(P<0.05);DCST1-AS1的表达与非小细胞肺癌患者TNM分期、组织分化程度、淋巴结转移及病理类型有关(P<0.05)。与对照组或si-NC组比较,si-DCST1-AS1组A549细胞中DCST1-AS1表达、OD值、克隆形成数、迁移细胞数、侵袭细胞数及Vimentin、N-cadherin表达降低(P<0.05),miR-29b、E-cadherin表达升高(P<0.05)。敲低miR-29b可明显减弱下调DCST1-AS1表达对A549细胞恶性生物学行为的影响。结论在非小细胞肺癌中DCST1-AS1呈高表达,敲低DCST1-AS1可能通过上调miR-29b表达抑制非小细胞肺癌细胞的恶性生物学行为。

Objective To detect the expression of DCST1-AS1 in non-small cell lung cancer and explore its effect on malignant biological behavior of cancer cells.Methods The tissues and corresponding adjacent tissues from 65 patients with non-small cell lung cancer were collected,and the normal human bronchial epithelial cells 16HBE and the non-small cell lung cancer cell lines(A549,H1299,H1650 and HCC827)were cultured in vitro.The expression levels of DCST1-AS1 and miR-29b in tissues and cells were detected by RT-qPCR assay,and the correlation between the DCST1-AS1 expression and the clinical characteristics of patients with non-small cell lung cancer were analyzed.A549 cells were divided into the control group,the si-NC group,the si-DCST1-AS1 group,the si-DCST1-AS1+anti-NC group and the si-DCST1-AS1+anti-miR-29b group.The cell proliferation was detected by CCK-8 assay and clone formation assay,the invasion and migration of cells were detected by Transwell;the expression of E-cadherin,Vimentin and N-cadherin was detected by Western blot.Results The expression of DCST1-AS1 increased and the expression of miR-29b decreased in non-small cell lung cancer tissues and cells(P<0.05).The expression of DCST1-AS1 was correlated with TNM stage,differentiated degree of tissue,lymph node metastasis and pathological types in non-small cell lung cancer patients(P<0.05).Compared with the control group or the si-NC group,the expression of DCST1-AS1,OD value,number of colony-forming cells,migration cells and invasion cells and the expression of Vimentin and N-cadherin in A549 cells of the si-DCST1-AS1 group decreased(P<0.05),while the expression of miR-29b and E-cadherin increased(P<0.05).Knocking down miR-29b could significantly reduce the effect of down-regulation of DCST1-AS1 expression on the malignant biological behavior of A549 cells.Conclusion DCST1-AS1 is highly expressed in non-small cell lung cancer,knocking down DCST1-AS1 may inhibit the malignant biological behavior of non-small cell lung cancer cells by up-regulating the expression of miR-29b.