鸡和兔血糖调节生理差异比较

Comparison of Physiological Differences in Blood Glucose Regulation between Chickens and Rabbits

为阐明禽类在高血糖情况下未表现出任何临床症状的内在原因,以鸡、家兔为研究对象,利用ELISA、高效液相色谱、液相色谱质谱检测等技术对两者葡萄糖调节生理差异进行比较。结果显示,ELISA检测鸡的糖化白蛋白显著高于兔,而糖化血红蛋白无显著性差异;鸡血清果糖胺含量与兔无显著性差异;鸡血清终末糖基化产物含量极显著低于兔;高效液相色谱检测结果显示,鸡的牛磺酸含量和游离氨基酸含量显著高于兔;液相色谱质谱检测结果显示,鸡的甲基乙二醛含量与家兔无显著性差异。以上研究结果表明,鸡无慢性高血糖所表现出的有害效应的主要原因是牛磺酸和部分游离氨基酸作为羰基清除剂降低果糖胺及甲基乙二醛的含量,从而导致糖基化终末产物的降低。

The fasting blood glucose concentrations of birds are about twice of themammals,and the birds have significant insulin resistance,but there is no significant diabetic complications,the reasons for the absense are unclear.In order to elucidate the intrinsic reasons for no clinical signs of hyperglycaemia inthe poultry,this study was conducted to compare the physiological differences in glucose regulation between chickens and rabbits using ELISA,high performance liquid chromatography and liquid chromatography mass spectrometry detection techniques.The results showed that glycosylated albumin was higher in chickens than in rabbits,while glycosylated haemoglobin was not significantly different;serum fructosamine levels inchickens were not significantly different from those in rabbits;and serum levels of advanced glycation end-products were significantly lower in chickens than in rabbits.The results of high performance liquid chromatography showed that the taurine content and free amino acid content of chickens were extremely significantly higher than those of rabbits;the results of liquid chromatography mass spectrometry showed that the methylglyoxal content of chickens was not significantly different from that of rabbits.In conclusion,the main reason forchickens to exhibite deleterious effects under the no chronic hyperglycaemiais that taurine and some free amino acids act as carbonyl scavengers to reduce the levels of fructosamine and methylglyoxal,and decrease the advanced glycation end-products.