摘要
目的:基于乙醇-硫酸铵双水相体系,制备独一味中苯乙醇苷类化合物。方法:以不同质量分数的乙醇-硫酸铵双水相体系提取独一味苯乙醇苷类化合物,以连翘酯苷B为标准品测定其含量。结果:当乙醇含量为33%、硫酸铵含量为17%时,苯乙醇苷得率达到92.08±0.09 mg/g。结论:双水相萃取优化过程简单、效果显著,可作为独一味有效成分提取的一种绿色新方法。
Objective:To prepare the phenylethanol glycosides in Lamiophlomis rotata based on the ethanolammonium sulfate two-phase aqueous system.Method:The Lamiophlomis rotata phenylethanol glycosides were extracted with different mass fractions of ethanol-ammonium sulfate two-phase system,and its content was determined with forsythiaside B as the standard.Results:When the content of ethanol was 33% and the content of ammonium sulfate was 17%,the yield of phenylethanol glycoside reached 92.08±0.09 mg/g.Conclusion:The optimization process of aqueous two-phase extraction is simple and the effect is remarkable,which can be used as a new green method for the extraction of Lamiophlomis rotata active ingredients.
作者
孙兆泉
乔茂旺
李彩云
徐丽
高禹涵
李新蕊
尚军
陈海娟
Sun Zhaoquan;Qiao Maowang;Li Caiyun;Xu Li;Gao Yuhan;Li Xinrui;Shang Jun;Chen Haijuan(Qinghai Normal University,College of Life Sciences,Qinghai Key Laboratory of Medicinal Animal and Plant Resources on the Qinghai-Tibet Plateau,Xining 810000,China;Institute of Plateau Science and Sustainable Development,Xining 81000,China)
出处
《青海科技》
2023年第5期77-81,共5页
Qinghai Science and Technology
基金
青海省重点研发与转化计划国际合作专项(2021-HZ-805)
青海省“昆仑英才·高端创新人才”项目(2020)。
关键词
独一味
乙醇-硫酸铵双水相
苯乙醇苷
紫外-可见分光光度法
Lamiophlomis rotata
Ethanol-ammonium sulfate aqueous two phase
Phenylethanol glycosides
UV-visible spectrophotometry
