基于CRITIC法优化大叶冬青瞬时高温灭菌工艺

Optimization of Instantaneous High-temperature Sterilization Process Before and After Sterilization of Ilex latifolia Based on CRITIC Method

为优化大叶冬青瞬时高温灭菌工艺,分析其对大叶冬青灭菌效果及质量的影响,采用正交试验法,研究灭菌前后大叶冬青1,1-二苯基-2-苦基肼(1,1-Diphenyl-2-picrylhydrazyl, DPPH)自由基清除率及多酚类化合物和皂苷类化合物含量的变化,以灭菌温度、灭菌时间、药材粒度为考察因素,以灭菌率、DPPH自由基清除率、多酚类、皂苷类化合物含量为评价指标,通过权重分析法(criteria importance though intercrieria correlation,CRITIC)确定指标相关性权重并计算综合评分。结果表明,按照综合加权法确定的权重系数是多酚类化合物含量25.78%、皂苷类类化合物含量47.22%、DPPH·清除率26.99%,最佳灭菌工艺条件为:灭菌温度160或170℃,灭菌时间10 s,粉碎粒度不大于24目。按优化工艺灭菌后的样品微生物限度检查均符合药典规定;灭菌前后样品的多酚、皂苷类化合物含量及DPPH自由基清除率均无明显变化,且样品多酚、皂苷类化合物的指纹图谱相似度≥0.9。综上所述,瞬时高温灭菌能有效杀灭大叶冬青中的微生物,且对质量无影响。

To optimize the instantaneous high temperature sterilization process of Ilex macrophylla and investigate its effect on the sterilization effect and quality of Ilex latifolia,the orthogonal experiment was used.The changes of 1,1-dipheny l-2-picrylhydrazyl(DPPH)free radical clearance rate and the contents of polyphenolic compounds and saponins in Ilex latifolia before and after sterilization.The instantaneous high temperature sterilization process of Ilex macrophylla was optimized,with the sterilization temperature,sterilization time and medicinal material particle size as the investigation factors,and the sterilization rate,DPPH·clearance rate,contents of polyphenols and saponins as the evaluation indicators.The correlation weight of indicators was determined by criteria importance though intercrieria correlation(CRITIC)method to calculate the comprehensive score.The results showed that,according to the comprehensive weighting method,the weight coefficient of polyphenols,saponins and of DPPH·clearance rate were 25.78%,47.22%and 26.99%,respectively.The optimal sterilization process was as follows:sterilization temperature 160~170℃,sterilization time 10 s,and grinding size no more than 24 mesh.The microbial limits of the samples after sterilization with the optimized process were in accordance with the provisions of pharmacopoeia.And compared with before sterilization,there were no significant changes in the contents of polyphenols,saponins and DPPH·clearance rate after sterilization,the fingerprints of polyphenols and saponins after sterilization of samples were similar with before sterilization,with similarity≥0.9.In conclusion,instantaneous high temperature sterilization could effectively kill the microorganisms in Ilex latifolia,and had no effect on the quality.