蒲公英提取物对凡纳滨对虾优势腐败菌群体感应及保鲜效果的影响

Effects of Taraxacum mongolicum Extract on Quorum Sensing System of Specific Spoilage Organism and Preservation of Litopenaeus Vannamei

目的旨在探究蒲公英提取物(Taraxacum mongolicum Extracts,TME)对凡纳滨对虾的优势腐败菌嗜水气单胞菌(Aeromonas hydrophila)群体感应(Quorum Sensing,QS)系统的抑制作用,并探究TME对凡纳滨对虾低温贮藏保鲜效果的影响。方法本研究采用菌株紫色杆菌(Chromobacterium violaceum,CV026)测定TME群体感应抑制活性,并在亚抑菌浓度下测定TME对嗜水气单胞菌群体感应系统的抑制作用;随后测定凡纳滨对虾储藏过程中的菌落总数、挥发性盐基氮(Total Volatile Base Nitrogen,TVB-N)、pH值、汁液流失率以及感官评分的变化。结果TME对嗜水气单胞菌的最小抑菌浓度(Minimum Inhibitory Concentration,MIC)值为32 mg/mL。在1/4 MIC浓度下,TME显著抑制了嗜水气单胞菌群体感应信号分子的产生,并显著抑制嗜水气单胞菌群集运动能力和蛋白酶活性(P<0.05),TME处理显著抑制了凡纳滨对虾储存过程中感官评分的降低和菌落总数、TVB-N、pH值、汁液流失率的上升(P<0.05)。结论蒲公英提取物通过调控腐败菌的QS系统,降低了腐败菌的致腐能力,有效延长了凡纳滨对虾的保质期。

The work aims to explore the inhibitory effect of Taraxacum mongolicum extracts(TME)on the quorum sensing(QS)system of Aeromonas hydrophila and the specific spoilage organism of Litopenaeus vannamei,and explore the preservation effect of TME on the refrigerated L.vannamei.The quorum-sensing inhibitory activity of TME was investigated by using Chromobacterium violaceum(CV026).The preservation effect of TME on L.vannamei and its inhibitory effect on the quorum sensing system of A.hydrophila were measured at the subminimal inhibitory concentration.The total number of colonies,volatile base nitrogen(TVB-N),pH value,drip loss rate and sensory score of L.vannamei during storage were determined.The minimum inhibitory concentration(MIC)value of TME against A.hydrophila was 32 mg/mL.At 1/4 MIC,TME significantly inhibited the production of quorum sensing signal molecules,and inhibited the mobility and protease activity of A.hydrophila(P<0.05).TME significantly inhibited the decrease of sensory score and the increase of the total number of colonies,TVB-N,pH value and drip loss of L.vannamei(P<0.05).TME can reduce the spoilage ability of spoilage bacteria and effectively prolong the shelf life of L.vannamei by regulating QS system of the bacteria.