药用红树木榄胚轴抗HBV化学成分的研究

Anti-HBV chemical constituents from the hypocotyl of pharmaceutical mangrove Bruguiera gymnorhiza

红树木榄(Bruguiera gymnorhiza)胚轴为京族常用治疗乙肝中草药。为研究木榄胚轴中化学成分及其抗乙型肝炎病毒(hepatitis B virus, HBV)活性,该文采用MTT法和实时荧光定量PCR方法测定木榄胚轴不同萃取部位抗HBV活性,使用现代色谱和波谱方法对活性萃取部位的化学成分进行分离鉴定,并测试获得的化学成分抗HBV活性。结果表明:(1)红树木榄胚轴的正丁醇萃取部位具有抗HBV活性;(2)从中分离了11个化合物,分别鉴定为尿嘧啶(1)、胸腺嘧啶(2)、腺嘌呤核苷(3)、oryzalactam (4)、正丁基-O-D-吡喃果糖苷(5)、nortetillapyrone (6)、(4R,6S)-4-methoxyl-2,3-dihydroaquilegiolide (7)、(4R,6S)-2-dihydromenisdaurilide (8)、没食子儿茶素(9)、1-(4-hydroxy-3-methoxy)-phenyl-2-[4-(1,2,3-trihydroxypropyl)-2-methoxy]-phenoxy-1,3-propandiol (10)和(-)-南烛木树脂酚-9-O-β-D-木吡喃糖苷(11),其中化合物4、5、7和8为首次从药用红树木榄中获得,化合物4具有抗HBV活性,其抑制率为23.59%。该研究结果丰富了木榄胚轴抗HBV化学成分。

Bruguiera gymnorhiza hypocotyl is a common marine herbal medicine of the Jing people used in the treatment of hepatitis B.Firstly,MTT and real-time fluorescence quantitative PCR methods were used to determine the anti-HBV activity of different extracted parts of B.gymnorhiza hypocotyl,the chemical constituents of the active extraction parts were isolated and identified using modern chromatographic techniques and spectroscopic methods,and in vitro screening model was employed to test the anti-HBV activity of obtained compounds.The results were as follows:(1)The n-butanol phase of the hypocotyl exhibited anti-HBV activity;(2)A total of 11 compounds were isolated and structurally identified,namely uridine(1),thymidine(2),adenosine(3),oryzalactam(4),n-butyl-O-D-fructopyranoside(5),nortetillapyrone(6),(4 R,6 S)-4-methoxyl-2,3-dihydroaquilegiolide(7),(4 R,6 S)-2-dihydromenisdaurilide(8),gallcatechin(9),1-(4-hydroxy-3-methoxy)-phenyl-2-[4-(1,2,3-trihydroxypropyl)-2-methoxy]-phenoxy-1,3-propandiol(10),and(-)-lyoniresinol-9-O-β-D-xylopyrano-side(11),among them,compounds 4,5,7 and 8 were firstly obtained from B.gymnorhiza,and Compound 4 showed anti-HBV activity with an inhibition rate of 23.59%.The study clarify the chemical composition of the anti-HBV of B.gymnorhiza hypocotyl.