超高效液相色谱-串联质谱法测定茶叶中灭草松及其代谢产物残留量

Determination of Residual Levels of Bentazone and Its Metabolites in Tea by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry

建立了超高效液相色谱-串联质谱法测定茶叶中灭草松及其代谢产物的快速检测方法。茶叶样品使用0.1%冰乙酸-甲醇混合溶液提取,上清液使用石墨化碳黑(GCB)、N-丙基乙二胺(PSA)、MgSO4组成的复合填料净化过滤后,经超高效液相色谱-串联质谱法测定,最后通过外标法定量。结果表明,在试验条件下,灭草松在0.001~0.010 mg/L含量范围内具有较好的线性关系,其代谢产物在0.0002~0.0020 mg/L含量范围内同样具有较好的线性关系,相关系数(R2)均大于0.995;本方法对灭草松的定量限为0.01 mg/kg,对两种代谢产物的定量限均为0.002 mg/kg。该方法操作简单、快捷、灵敏、精密度高、成本低,能够满足茶叶中灭草松及代谢物的检测需求。

A rapid method for the determination of bentazone and its metabolites in tea by ultra-high performance liquid chromatography-tandemmass spectrometry was established.The tea samples were extracted with 0.1%glacial acetic acidmethanol,and the liquid supernatant was purified and filtered by PSA,GCB and MgSO4,which was determined by high performance liquid chromatography-mass spectrometry and quantified by external standardmethod.The results showthat under the experimental conditions,the bentazone had a good linear relationship in the range of 0.001~0.010 mg/L,and its metabolites also had a good linear relationship in the range of 0.0002~0.0020 mg/L,with correlation coefficients(R2)greater than 0.995.The quantitative limits for bentazone and its metabolites were 0.01 mg/kg and 0.002 mg/kg,respectively.The method is simple,fast,sensitive,with high precision and low cost,and can meet the requirements for the detection of bentazone and its metabolites in tea.