摘要
建立了超高效液相色谱-串联质谱法测定茶叶中灭草松及其代谢产物的快速检测方法。茶叶样品使用0.1%冰乙酸-甲醇混合溶液提取,上清液使用石墨化碳黑(GCB)、N-丙基乙二胺(PSA)、MgSO4组成的复合填料净化过滤后,经超高效液相色谱-串联质谱法测定,最后通过外标法定量。结果表明,在试验条件下,灭草松在0.001~0.010 mg/L含量范围内具有较好的线性关系,其代谢产物在0.0002~0.0020 mg/L含量范围内同样具有较好的线性关系,相关系数(R2)均大于0.995;本方法对灭草松的定量限为0.01 mg/kg,对两种代谢产物的定量限均为0.002 mg/kg。该方法操作简单、快捷、灵敏、精密度高、成本低,能够满足茶叶中灭草松及代谢物的检测需求。
A rapid method for the determination of bentazone and its metabolites in tea by ultra-high performance liquid chromatography-tandemmass spectrometry was established.The tea samples were extracted with 0.1%glacial acetic acidmethanol,and the liquid supernatant was purified and filtered by PSA,GCB and MgSO4,which was determined by high performance liquid chromatography-mass spectrometry and quantified by external standardmethod.The results showthat under the experimental conditions,the bentazone had a good linear relationship in the range of 0.001~0.010 mg/L,and its metabolites also had a good linear relationship in the range of 0.0002~0.0020 mg/L,with correlation coefficients(R2)greater than 0.995.The quantitative limits for bentazone and its metabolites were 0.01 mg/kg and 0.002 mg/kg,respectively.The method is simple,fast,sensitive,with high precision and low cost,and can meet the requirements for the detection of bentazone and its metabolites in tea.
作者
段联勃
柯顺川
符海霞
杨丽蓉
曹士先
徐杰
DUAN Lianbo;KE Shunchuan;FU Haixia;YANG Lirong;CAO Shixian;XU Jie(Wuyi Star Tea Industrial Co.,Ltd.,Wuyishan 354300,China;Fujian Enterprise Technology Center,Wuyishan 354300,China)
出处
《中国茶叶》
2023年第12期40-45,53,共7页
China Tea
基金
福建省区域发展项目(2021N3010)。