响应面法优化榆黄蘑熟料袋栽菌丝体培养条件

Optimization of mycelium culture conditions for bag cultivation of Pleurotus citrinopileatus by response surface methodology

以榆黄蘑为试验材料,基于最佳熟料袋栽配方进行培养条件的单因素试验,根据单因素试验结果,以菌丝生长速度为响应值,采用Box-Behnken中心组合原理进行响应面优化试验,对菌丝培养中的接种量、pH、温度和培养料含水量4个不同培养条件进行优化研究。结果表明,4个因素对榆黄蘑菌丝生长速度的影响大小为温度>接种量>pH>培养料含水量,最佳的培养条件为接种量7%、pH 6.5、温度26℃、培养料含水量65%,该培养条件下榆黄蘑菌丝生长速度可达9.99 mm/d。

Pleurotus citrinopileatus was used as the test material,and the single factor test of the culture conditions was performed based on the best bag culture with sterilized raw materials.On the basis of the single factor experiment,the response surface optimiza⁃tion experiment was carried out with the mycelium growth rate as the response value by using the Box-Behnken central combination principle,and the optimization research was carried out on four different culture conditions in mycelium culture,including inoculum volume,pH,temperature and water content of culture material.The results showed that the influence of the four factors on the myceli⁃um growth rate of Pleurotus citrinopileatus was temperature>inoculum volume>pH>water content of culture material.The optimal culture condition was inoculum volume of 7%,pH of 6.5,temperature of 26℃,and water content of culture material of 65%.Under this culture condition,the mycelium growth rate of Pleurotus citrinopileatus could reach 9.99 mm/d.