牦牛CAPS基因克隆及生物信息学分析

Cloning and Bioinformatics Analysis of Calcyphosine Gene(CAPS)in Yak

钙磷蛋白(calcyphosine,CAPS)是一种钙结合蛋白,对维护生物体内钙磷平衡起着重要作用。本研究克隆了牦牛CAPS基因的CDS区序列,分析了其核苷酸序列,并进行了生物信息学分析。结果表明,牦牛CAPS基因CDS区全长570 bp,编码189个氨基酸;编码蛋白的分子式为C_(905)H_(1434)N_(266)O_(297)S_(8),分子量为21049.43 Da,理论等电点为4.69,脂溶指数为75.29,不稳定指数为37.52,是疏水性稳定蛋白;系统进化分析表明,牦牛CAPS基因CDS区与普通牛和瘤牛的基本一致,与水牛、绵羊和猪的序列一致性较高。本研究为深入研究牦牛CAPS的生理功能提供了参考资料。

As a calcium-binding protein,calcyphosine(CAPS)plays an important role in maintaining the balance of calcium and phosphorus in vivo.In this study,the CDS region of yak CAPS gene was cloned,its nucleo-tide sequence was analyzed,and bioinformatics analysis was carried out.Results showed that the CDS region of CAPS gene was 570 bp long and encoded 189 amino acids.The formula,molecular weight,theoretical pI,aliphat-ic index,instability index of the encoded protein was C_(905)H_(1434)N_(266)O_(297)S_(8),21049.43 Da,4.69,75.29 and 37.52,respectively,which suggested it was a stable fat-soluble protein.Phylogenetic analysis showed that the CDS region of CAPS gene in yak had the highest similarity with that of common cattle and Zebu,with higher simi-larity with buffalo,sheep and pig.This study provided reference datafor further study on the physiological function of CAPS in yak.