miR-642a-3p对藻蓝色素抑制非小细胞肺癌活性的调控作用

The Regulation Function of miR-642a-3p on the Inhibitory Activity of Non-small Cell Lung Cancer Mediated by Phycocyanin

藻蓝色素(PC)是一种来自海洋藻类的天然色素蛋白复合体,也是一类重要的食品功能因子,具有抗肿瘤、抗炎、增强免疫等生理活性,被广泛应用于功能性食品、医药及化妆品等领域。藻蓝色素对非小细胞肺癌(NSCLC)细胞活性的抑制作用已被证实,然而其机制尚不明确。本研究以miR-642a-3p为切入点,探究藻蓝色素抑制NSCLC细胞活性的机制。试验结果表明,藻蓝色素能够上调4种NSCLC细胞(A549、H1299、H460和LTEP-a2)中miR-642a-3p的表达水平。过表达干预细胞中的miR-642a-3p水平能够显著抑制4种细胞的体外增殖、迁移和非锚定生长能力。此外,转染miRNA抑制剂干预miR-642a-3p的表达,在一定程度上增强了细胞的活性。本研究初步揭示miR-642a-3p上调表达参与藻蓝色素抑制NSCLC细胞活性的过程,为藻蓝色素的深入研究和利用提高科学依据。

Phycocyanin(PC)is a kind of natural pigment protein complex derived from marine algae.It is also a type of important food functional factor with anti-cancer,anti-inflammatory,and immune-enhancing activities,and has been widely used in functional food,medicine and cosmetics.Phycocyanin has been proven to inhibit the activity of non-small cell lung cancer(NSCLC)cells,but the underlying regulatory mechanism is not fully understood.This study focused on miR-642a-3p,and investigated the potential regulating mechanism of phycocyanin in NSCLC cells.The results showed that phycocyanin upregulated the expression level of miR-642a-3p in four NSCLC cells(A549,H1299,H460 and LTEP-a2).Overexpression of miR-642a-3p levels significantly inhibited the proliferation,migration and unanchored growth of four cells in vitro.In addition,transfection of miRNA inhibitors to interfere with the expression of miR-642a-3p could enhance cell viability to a certain extent.This study preliminarily revealed that the up-regulation expression of miR-642a-3p was involved in the regulation of inhibiting NSCLC cell viabilities by phycocyanin,which could lay a scientific theoretical basis for the in-depth study and utilization of phycocyanin.