两性离子聚合物改性抗污染棉签拭子检测牛奶中的沙门氏菌

Zwitterionic Polymer-modified Cotton Swabs for Rapid Detection of Salmonella in Milk

为了高效快速检测牛奶中的沙门氏菌,提高抗污染生物传感器的检测灵敏度,本研究基于两性离子聚甲基丙烯酸磺基甜菜碱(SBMA)改性棉签拭子获得抗污染性能,构建以“蓝色乳胶微球/适配体”为比色信号探针的传感检测平台,对牛奶样品中沙门氏菌进行快速检测。本方法对沙门氏菌的线性检测范围为10^(3)~10^(7)CFU/mL,检测限为117.80 CFU/mL。整个检测结果能在20 min内完成,既可以利用肉眼进行定性结果测定,也可以配合使用便携式色差仪与智能手机结合的检测装备定量读出检测结果,操作简单、方便,为现场快速检测沙门氏菌提供可能。

In order to efficiently and rapidly detect Salmonella in milk,and improve the sensitivity of the anti-fouling biosensor.In this study,amphoteric poly(sulfobetaine methacrylate)(SBMA)-modified swab was used to obtain the anti-fouling property,and constructed a sensing platform with'blue latex microspheres/adaptor'as the colorimetric signal probe for the rapid detection of Salmonella in milk samples.The linear detection of Salmonella was 10^(3)-10^(7)CFU/mL,and the detection limit was 117.80 CFU/mL.The detection is completed in 20 min,which can be qualitative to the naked eye,can be combined with the detection of portable colorimeter smartphones,can be measured and read,and is easy to operate,which is also a rapid detection of Salmonella.