一种固有抗菌的黏附性可注射水凝胶用于牙周炎骨缺损治疗的初步研究

An inherent antibacterial adhesive injectable hydrogel for the treatment of periodontitis bone defects

目的构建并制备具有固有抗菌黏附性的聚乙二醇(PEG)-聚赖氨酸(PLL)注射型水凝胶,探究其各项性能及在牙周炎治疗中的效果。方法ε-聚赖氨酸通过酰胺化反应与四臂聚乙二醇琥珀酰亚胺戊二酸酯交联获得水凝胶。通过傅里叶变换红外光谱、扫描电子显微镜等对材料进行表征分析,考察不同配比材料的抗菌性能、生物相容性、凝胶化时间、溶胀性和力学性能,确定最佳配比;评估其黏附强度;体内构建大鼠牙周炎模型,验证PEG-PLL水凝胶在牙周炎中的疗效。结果成功构建了PEG-PLL水凝胶,其中当PLL浓度为20 mg/mL时水凝胶具有良好的抗菌性能、力学性能、凝胶化时间及较低的溶胀率,且无明显的细胞毒性,产生约15 kPa的黏附力。动物体内实验证实该水凝胶可有效阻止牙周炎骨缺损的进一步发展,促进骨缺损修复。结论PEG-PLL水凝胶具有良好的固有抗菌、生物相容性,并有一定的组织黏附性,可促进牙周炎骨缺损的修复。

Objective To prepare polyethylene glycol(PEG)-polylysine(PLL)injectable hydrogel with inherent antibacterial adhesion,and explore its various functions and effects in the treatment of periodontitis.Methods ε-polylysine was crosslinked with 4-armPEG-SG to obtain hydrogel through amidation reaction.The materials were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy.The antibacterial properties,biocompatibility,gel time,swelling and mechanical properties of the materials with different ratios were investigated to determine the best ratio.The study also evaluated the hydrogel's adhesion strength.A rat model of periodontitis was constructed to observe the therapeutic effects of PEG-PLL hydrogel.Results PEG-PLL hydrogel was successfully constructed.When the concentration of PLL was 20 mg/mL,the hydrogel had good antibacterial properties,mechanical property,gel time,low swelling rate,and no obvious cytotoxicity.It can produce adhesion strength of about 15 kPa.In vivo experiments proved that the hydrogel can effectively prevent the further development of periodontal bone defects and promote the repair.Conclusion PEG-PLL hydrogel has good inherent antibacterial activity,biocompatibility and tissue adhesion,which can promote the repair of periodontal bone defects.