邻联甲苯胺法和胶体金法测定粪便潜血的比较及联合检测的意义

Comparison of o-toluidine method and colloidal glod method for determination of fecal occult blood and the significance of joint detection

目的用邻联甲苯胺法和胶体金法对粪便潜血进行检测,探讨邻联甲苯胺法和胶体金法联合检测粪便潜血的临床意义。方法收集该院消化道出血患者粪便51例(试验组),健康体检者粪便53例(对照组),对同一份标本同时进行邻联甲苯胺法和胶体金法检测,比较分析粪便潜血检测的两种方法结果的差异,探讨两种方法的优缺点。结果试验组患者粪便中的阳性率为100%(51/51),对照组粪便中的阳性率为25.6%(14/53);胶体金法在试验组患者粪便中的阳性率为65.2%(33/51),在对照组粪便中的阳性率为0%(0/53),研究发现邻联甲苯胺法阳性率均高于胶体金法,比较差异有统计学意义(P<0.01)。结论粪便潜血检测,邻联甲苯胺法有较高的假阳性率,但敏感性较高。胶体金法不受饮食,药物的影响,抗干扰能力好,特异性高,但敏感性不如邻联甲苯胺法,如果联合使用这两种方法,粪便潜血检测的敏感性和准确性可得到有效保证,更能提高对消化道出血性疾病的准确诊断。

Objective To detect fecal occult blood by o-toluidine method and colloidal gold method,analyze the clinical significance of o-toluidine method and colloidal gold method in the detection of fecal occult blood.Methods The stool of 51 patients with digestive tract bleeding(experimental group)and 53 patients with normal physical examination(control group)were collected in the hospital.The stool of the same specimen was detected by o-toluidine method and colloidal gold method at the same time.The difference of the results of the two methods of fecal occult blood detection was compared and analyzed,and the advantages and disadvantages of the two methods were discussed.Results The positive rate of o-toluidine method was 100%(51/51)in the experimental group,and 25.6%(14/53)in the control group with normal physical examination.The positive rate of colloidal gold method was 65.2%(33/51)in the feces of experimental group,and 0%(0/53)in the feces of control group.It was found that the positive rate of o-toluidine method was higher than that of colloidal gold method,and the difference was significant(P<0.01).Conclusion In the detection of fecal occult blood,o-toluidine method has higher false positive rate,but higher sensitivity.Colloidal gold method is not affected by diet and drugs,has good anti-interference ability and high specificity,but its sensitivity is not as good as o-toluidine method.If the methods are used together,the sensitivity and accuracy of fecal occult blood test can be effectively guaranteed,and it can improve the accurate diagnosis of digestive hemorrhagic diseases.