基于组学技术分析肺炎支原体肺炎患儿血清生物标志物

Analysis of serum biomarkers in children with Mycoplasma pneumoniae pneumonia based on histological techniques

目的:采用代谢组学分析肺炎支原体肺炎儿童血清中代谢物的变化,以期筛选出与肺炎支原体肺炎相关的潜在生物标志物,并进行相关通路分析。方法:选取2022年5月至2022年7月内蒙古医科大学附属医院儿科收治的肺炎支原体肺炎患儿为病例组,同时选取年龄匹配的健康体检儿童为对照组。采集研究对象晨起空腹静脉血2 mL,利用UPLC-Q-TOF-MS方法分析血清中代谢产物,并进行通路富集分析。结果:正、负离子模式共筛选出243种代谢物,差异代谢物有19种,与对照组相比,病例组升高6种物质,降低13种物质。通路富集分析结果显示,代谢通路包括氨基酸的生物合成、胆固醇代谢及鞘脂信号通路等。结论:儿童肺炎支原体肺炎患者与正常儿童的血清非靶向代谢组存在差异,磷脂酰胆碱对肺炎支原体肺炎具有一定的预测价值。

Objective:To analyze the changes of metabolites in serum of children with Mycoplasma pneumoniae pneumonia by metabolomics,in order to screen out potential biomarkers related to Mycoplasma pneumoniae pneumonia and analyze related pathways.Methods:Children with mycoplasma pneumoniae pneumonia admitted to the Department of Pediatrics,the Affliated Hospital of Inner Mongolia Medical University from May 2022 to July 2022 were selected as the case group,and age-matched healthy children were selected as the control group.A total of 2 mL fasting venous blood was collected from the subjects in the morning.UPLC-Q-TOF-MS method was used to analyze the metabolites in serum,and pathway enrichment analysis was performed.Results:A total of 243 metabolites were screened by positive and negative ion models,and 19 metabolites were different.Compared with the control group,6 substances increased and 13 substances decreased in the case group.Pathway enrichment analysis showed that metabolic pathways included amino acid biosynthesis,cholesterol metabolism and sphingolipid signaling pathway.Conclusion:The serum non-targeted metabolome of children with Mycoplasma pneumoniae pneumonia is different from that of normal children,and phosphatidylcholine has some predictive value for Mycoplasma pneumoniae pneumonia.