利用转录组分析光照对金耳子实体转色的影响

Transcriptome Analysis Revealed Impact of Light Quality on Coloration of Naematelia aurantialba Fruiting Bodies

以生长在黑暗、白光、蓝光下的金耳(Naematelia aurantialba)子实体为材料,利用转录组分析光照对金耳子实体转色的影响。差异表达基因分析结果表明:白光诱导的转色子实体与黑暗处理的未转色子实体相比,差异表达基因总数为1 797个(1 308个上调,489个下调);蓝光诱导的转色子实体与黑暗处理的未转色子实体相比,差异表达基因总数为1 617个(1 056个上调,561个下调);蓝光诱导的转色子实体和白光诱导的转色子实体相比,差异表达基因总数为152个(74个上调,78个下调)。GO功能富集分析表明:与黑暗处理未转色子实体相比,蓝光或白光诱导的转色子实体中大部分差异表达基因上调,且主要富集在翻译、肽类代谢、核糖体结构成分、结构分子活性等过程;蓝光和白光诱导的子实体中,差异表达基因主要富集在呼吸调控、跨膜运输及膜组分等相关过程。KEGG代谢途径富集分析表明:参与核糖体、氨基酸生物合成及2-氧羧酸代谢等途径的相关基因在子实体转色过程中大部分上调表达。根据KEGG代谢途径富集分析,筛选到9条与类胡萝卜素生物合成、核黄素代谢等相关的色素代谢途径及差异表达基因。从蓝光和黑暗比较组中的类胡萝卜生物合成途径、核黄素代谢及其与色素合成相关的上游代谢途径中筛选出9个上调差异表达基因进行qRT-PCR验证,结果显示9个基因表达趋势与转录组结果一致。研究结果可为金耳子实体转色机制解析、关键功能基因挖掘及栽培过程中的光照调控提供参考。

Transcriptome analysis was used to investigate the effects of light quality on coloration of Naematelia aurantialba fruiting bodies.N.aurantialba fruiting bodies were cultivated under dark(D_2),white light(W_2),and blue light(B_2)conditions,respectively.Differential gene analysis revealed that 1797 genes were differentially expressed between W_2 and D_2.Among these genes,1308 were up-regulated and 489 were down-regulated.There were 1617 differentially expressed genes(DEGs)between B_2 and D_2,among which 1056 were up-regulated and 561 were down-regulated.In comparison with white light induction,blue light induction resulted in differential expression of 152 genes,with 74 up-regulated and 78 down-regulated.GO enrichment analysis showed that most DEGs as compared with D_2 were up-regulated and mainly enriched in translation,peptide metabolism,ribosome structural components,structural molecules activity and other processes induced by blue or white light.Compared with W_2,the DEGs of B_2 were mainly enriched in respiratory regulation,transmembrane transport,and membrane components.KEGG enrichment analysis showed that genes involved in ribosome,amino acid biosynthesis,and 2-oxoacid metabolism pathways were up-regulated during the coloration process.According to KEGG metabolic pathway analysis,nine DEGs were identified to be associated with pigment metabolism,e.g.carotenoid biosynthesis and riboflavin metabolism.The qRT-PCR results of the nine genes were consistent with the transcriptome data.These results provided a reference for understanding the underlying mechanism of N.aurantialba coloration,identification of key functional genes,and light condition regulation during cultivation.