转Pofst3糙皮侧耳菌株农艺性状和胞外酶活性分析

Agronomic Traits and Extracellular Enzyme Activities of Pofst3 Transgenic Pleurotus ostreatus

通过袋栽实验比较野生型、Pofst3过表达型和Pofst3反义沉默型糙皮侧耳(Pleurotus ostreatus)菌株(分别记为Ⅰ、Ⅱ、Ⅲ)的菌丝生长速度、原基数量、子实体数量、菌柄直径、菌盖直径等农艺性状;测定其漆酶、木聚糖酶、纤维素酶活性。结果表明:Ⅰ、Ⅱ、Ⅲ菌丝生长速度差异不显著;与Ⅰ相比,Ⅲ的原基和子实体数量多,菌柄直径大,菌盖直径小,原基和子实体中Pofst3的相对表达量低;与Ⅰ、Ⅱ相比,Ⅲ的漆酶、木聚糖酶及纤维素酶活性较高;Pofst3对糙皮侧耳发育的影响可能与其胞外酶活性相关。

Through bag cultivation experiments,wild type(typeⅠ),Pofst3 overexpression(typeⅡ),and Pofst3 antisense silencing(typeⅢ)Pleurotus ostreatus were compared for agronomic traits,including mycelial growth rate,number of primordia and fruiting bodies,stipe diameter and pileus diameter.These strains were also measured for activities of laccase,xylanase,and cellulase.The results showed that there was no significant difference in mycelial growth rate between the three strains.Compared with typeⅠ,typeⅢhad more primordia,more fruiting bodies,greater stipe diameter,smaller pileus diameter and expressed a lower level of Pofst3 in primordium and fruiting body.Compared with typeⅠand typeⅡ,typeⅢshowed higher activities of laccase,xylanase,and cellulase,suggesting that the effects of Pofst3 on the development of P.ostreatus may be associated with its extracellular enzyme activities.