超声辐照对载盐霉素钠纳米气泡的药物释放作用

Influence of ultrasonic irradiation on drug release effect of salinomycin sodium-loaded nanobubbles

目的:观察空白纳米气泡和载盐霉素钠纳米气泡的物理性质,探讨不同超声辐照时间和强度对载盐霉素钠纳米气泡的药物释放效果。方法:应用机械震荡法制备空白纳米气泡和载盐霉素钠纳米气泡。检测纳米气泡的稳定性、粒径大小,显微镜下观察纳米气泡的形态,观察两种纳米气泡大小随时间改变发生的变化。观察不同超声辐照强度和时间对载盐霉素钠纳米气泡释放作用的影响,CCK-8法观察载盐霉素钠纳米气泡对前列腺癌PC3细胞活力的影响。结果:显微镜下观察两种纳米气泡大小均一,分布均匀。空白纳米、载盐霉素钠纳米气泡第0天~第14天大小差异均无统计学意义(P>0.05)。同一辐照强度,与0 s相比,吸光度(OD)值均下降(P<0.05)。同一辐照时间(5~25 s),与2.5 W/cm 2相比,OD值均升高(P<0.05)。结论:本研究制备的两种纳米气泡物理性质良好。超声辐照可以帮助载盐霉素钠纳米气泡中盐霉素钠的释放,为前列腺癌的治疗提供了一种思路。

Objective:To observe the physical properties of blank nanobubbles and salinomycin sodium-loaded nanobubbles,and to investigate influence of different ultrasonic irradiation time and intensity on drug release effect of salinomycin sodium-loaded nanobubbles.Methods:Mechanical shock method was used to prepare blank and salinomycin sodium-loaded nanobubbles that were measured for their stability and particle size as well as microscopically observed for their morphology,size change of two types of nanobubbles over time and the influence of different ultrasonic irradiation time and intensity on drug release effect of salinomycin sodium-loaded nanobubbles.Cell counting kit-8(CCK-8)was then used to detect the effect of salinomycin sodium-loaded nanobubbles on the viability of prostate cancer PC3 cells.Results:Two types of nanobubbles had uniform size and distribution under microscope.There was no significant difference in the size of the two types of nanobubbles on day 0 and day 14(P>0.05).Under the same irradiation intensity,the optical density(OD)value was decreased compared with that at 0s(P<0.05).At the same irradiation time(5-25 s),the OD value was increased compared with that irradiated at 2.5 W/cm 2(P<0.05).Conclusion:Two types of nanobubbles have good physical properties.Ultrasound irradiation can help release salinomycin sodium from salinomycin sodium-loaded nanobubbles,which may provide an approach to the treatment of prostate cancer.