利血平对脊髓小脑共济失调3型(SCA3)细胞模型的影响

Effect of reserpine on spinocerebellar ataxia type 3(SCA3)cell model

目的 阐明利血平对脊髓小脑共济失调3型(SCA3)细胞模型的潜在作用。方法 通过核质分离、免疫荧光、Western blotting、微孔过滤和细胞活性等实验探索利血平对SCA3发病过程中ataxin-3核质转运、ataxin-3可溶性蛋白水平、蛋白包涵体、细胞活性等一系列病理变化的影响。结果 利血平对ataxin-3核质转运和可溶性蛋白水平没有显著性影响,但是增加了ataxin-3形成的包涵体数量,并且增加了细胞毒性。结论 利血平通过增加包涵体数量导致SCA3细胞活性减弱,为老药新用发现提供了理论依据。

Objective To clarify the potential effect of reserpine on spinocerebellar ataxia type 3(SCA3)cell model.Methods The effects of reserpine on ataxin-3 nuclear and cytoplasmic transport,ataxin-3 soluble protein level,protein inclusion body and cell viability during the pathogenesis of SCA3 were investigated by nucleocytoplasmic separation,immunofluorescence,Western blotting,filter trap and CCK-8 methods.Results Reserpine had no significant effect on the nucleocytoplasmic transport and soluble protein level of ataxin-3,but increased the number of inclusion bodies formed by ataxin-3 and increased cytotoxicity.Conclusion Reserpine decreases the SCA3 cell viability by increasing the number of inclusion bodies,which provides a theoretical basis for the new discovery of old drugs.