EphA2与B7-H3在结直肠癌组织中表达及其与临床病理和预后的相关性

Expression of EphA2 and B7-H3 and their correlations with clinicopathological parameters and the prognosis of colorectal cancer patients

目的探讨促红细胞生成素肝细胞A2(Erythropoietin-producing hepatocellular A2,EphA2)与免疫调节分子B7-H3在结直肠癌(colorectal cancer,CRC)中的表达及其临床预后价值。方法收集行手术治疗的110例CRC患者的石蜡包埋组织,通过免疫组织化学染色检测CRC组织中EphA2与B7-H3的表达,分析两者与CRC临床病理参数的关系。此外,通过Kaplan-Meier生存曲线和Cox风险比例模型评估EphA2与B7-H3表达在CRC患者中的预后意义。结果在所分析的110例CRC样本中,65.5%(72/110)呈现EphA2高表达,而61.8%(68/110)有B7-H3高表达。EphA2高表达者肿瘤直径更大(P=0.043)、淋巴结转移(P=0.034)和淋巴血管侵犯较低表达者更为频繁(P=0.026)。此外,与低表达组相比,B7-H3高表达者具有更为进展的T分期(P=0.024)和更高的淋巴结转移率(P=0.045)。生存分析显示,EphA2高表达与低表达患者的5年总体生存(overall survival,OS)率分别为43.9%和66.1%(P=0.014)。B7-H3高表达组的5年OS率显著低于低表达组,分别为33.2%和66.7%(P=0.028)。单、多变量Cox回归分析表明EphA2高表达(HR=2.576,95%CI:1.040-6.384,P=0.041)、B7-H3高表达(HR=2.305,95%CI:1.051~5.057,P=0.037)是CRC患者的独立预后因素。结论EphA2和B7-H3高表达与侵袭性生物学特征及不良预后显著相关,二者或许是结直肠癌患者可靠的预后分子标志。

Objective To investigate the expression of Erythropoietin-producing hepatocyte A2(EphA2)and immunomodulatory molecule B7-H3 and their prognostic value for colorectal cancer(CRC)patients.Methods A total of 110 paraffin-embedded samples were collected from CRC patients who received surgical resection.The expression levels of EphA2 and B7-H3 protein in CRC tissues were detected by immunohistochemical staining,and their correlations to clinicopathological parameters were analyzed.In addition,the prognostic significance of EphA2 and B7-H3 expression for CRC patients were evaluated by the Kaplan-Meier method and the Cox proportional hazards model.Results Of 110 CRC samples,65.5%(72/110)had a positive expression of EphA2 and 61.8%(68/110)had a positive expression of B7-H3.Patients with high expression of EphA2 had larger tumor size(P=0.043),more frequent lymph node metastasis(P=0.034)and lymphovascular invasion(P=0.026).Compared with those with low expression,patients with high B7-H3 expression had more advanced T stage(P=0.024)and higher rate of lymph node metastasis(P=0.045).Survival analysis indicated that the 5-year overall survival(OS)rates of patients with high and low EphA2 expression were 43.9%and 66.1%,respectively(P=0.014).The 5-year OS rate of patients with high B7-H3 expression was significantly lower than that of low B7-H3 expression(33.2%vs 66.7%,P=0.028).The data of the univariate and multivariate Cox regression analysis demonstrated that high expression of EphA2(hazard ratio[HR]=2.576,95%confidence interval[CI]:1.040-6.384,P=0.041)and B7-H3(HR=2.305,95%CI:1.051-5.057,P=0.037)were independent prognostic factors of CRC patients.Conclusion High expression of EphA2 and B7-H3 were significantly correlated with aggressive biological characteristics and poor prognosis of CRC patients,and they might be valuable prognostic biomarkers.