甜瓜CmVDAC基因家族的鉴定及转录分析

Identification and transcriptional analysis of CmVDAC gene family in Cucumis melo

电压依赖性阴离子通道蛋白(VDAC)是线粒体的“开关”,决定线粒体的命运,对甜瓜VDAC家族成员进行鉴定,对基因结构、进化、亚细胞定位及转录模式进行了分析。结果表明,甜瓜全基因组共鉴定出7个VDAC家族成员,命名为CmVDAC1~7,分别分布在6条染色体上,含有5~10个内含子。该家族编码的蛋白质大多为弱碱性、亲水性氨基酸,氨基酸数量为276~380 aa。系统进化分析结果表明,CmVDAC蛋白成员分为4个亚族,且亚细胞定位预测显示,除Cm VDAC7蛋白外,其他CmVDAC家族蛋白均定位到线粒体。启动子序列分析发现,CmVDAC启动子序列包含了调控植物生长发育和逆境胁迫响应两个方面的顺式作用元件,说明Cm VDAC基因可能参与植物生长发育和逆境胁迫响应。组织特异性表达分析显示,CmVDAC基因在甜瓜的根、叶、果实、雌花和雄花都有不同程度的表达,表明CmVDAC基因可能与植物生长发育有关,且在不同组织部位发挥不同的生物学功能。枯萎病菌胁迫下的基因转录模式表明,CmVDAC可以响应甜瓜枯萎病菌的侵染;对CmVDAC4基因进行荧光定量PCR,结果表明CmVDAC4可以响应白粉病菌的侵染,且在抗病甜瓜材料的表达量显著高于其在感病甜瓜材料的表达。本研究为CmVDAC基因在甜瓜生长发育和逆境胁迫的功能提供参考,同时也为抗病育种提供理论指导。

Abstact:The voltage-dependent anion channel protein(VDAC channel protein)is the“switch”of mitochondria,which determine the fate of mitochondria and play an important role in plant growth and development and response to biological or abiotic stresses.In this paper,the VDAC family of melon were identified,and the gene structure,evolution,subcellular localization and transcription patterns were analyzed.The results showed that a total of 7 VDAC family members,named Cm VDAC1–7,were identified in melon genome,which were distributed on 6 chromosomes and contained 5 to10 introns.Most of the proteins encoded by this family are weakly alkaline and hydrophilic amino acids,with amino acids ranging from 276 to 380 aa.Phylogenetic analysis showed that Cm VDAC proteins were divided into four subfamilies,and the subcellular localization prediction showed that except for Cm VDAC7 protein,the other Cm VDAC family proteins were all localized to mitochondria.Promoter sequence analysis showed that Cm VDAC promoter sequence contains cis-acting elements that regulate plant growth and development and stress response element,suggesting that Cm VDAC may be invloved in palnt growth and development and stress response.Tissue-specific expression showed that Cm VDAC were expressed in different degrees in roots,leaves,fruits,female and male flowers of melon,suggesting that Cm VDAC may be related to plant growth and development,and play different biological functions in different tissue parts.The transcriptional pattern of genes under fusarium wilt stress showed that Cm VDAC could response to fusarium wilt infection.Quantitative PCR of Cm VDAC4 showed that Cm VDAC4 could response to powdery mildew pathogen infection,and its expression level in disease–resistant melon was significantly higher than that in diseasesusceptible melon.The results of this study can provide reference for the function of Cm VDAC in melon growth and development and stress resistance,and also provide theoretical guidance for disease resistance breeding.