光热显微成像:一种免标记、高分辨的成像技术

Photothermal Microimaging: A Non-Invasive and High-Resolution Imaging Technique

高分辨光学成像技术对生物学、生命科学以及材料科学的发展都有重要意义。主流的高分辨光学成像技术依赖荧光成像,但是荧光高分辨成像不能揭示分子特异性信息且极易导致细胞毒性。而光热显微成像技术是一种兼具成分分辨和非侵入性的新兴技术,能够填补高分辨荧光成像的缺陷,具有极大的应用前景。对光热显微镜技术的实现原理、技术发展进行简要介绍,并总结提高中红外光热显微成像的探测极限和分辨率的措施,旨在为进一步推动高灵敏度和超分辨光热成像技术的发展提供借鉴思路。

Super-resolution optical imaging technology has significant implications for biology,life science,and materials science.The mainstream technology to achieve super-resolution optical imaging relies on fluorescence imaging,but fluorescence-based super-resolution imaging cannot reveal molecular-specific information and causes cytotoxicity to living cells.In contrast,photothermal microscopy is a promising analytical technique that allows noninvasive imaging of molecular bonds.Therefore,photothermal microscopy can overcome the inherent limitations of super-resolution fluorescence imaging,making it an attractive option with excellent application prospects.This review discusses the theoretical basis of photothermal microscopy,development of the imaging technique,and the methodological developments that improve the detection limit and spatial resolution.We further provide future perspectives for promoting the development of high-sensitivity and super-resolution photothermal imaging technology.