野生金线莲根际土壤微生物分析

Microbial Analysis of Soil Microorganisms of Wild Anoectochilus formosanus

为分析野生金线莲根际土壤微生物的种类和丰度,优化金线莲仿生态栽培土壤条件,本研究采用Illumina NovaSeq高通量测序技术对6个样本福建金线莲根际土壤微生物16S区域文库进行双末端测序,分析金线莲根际土壤微生物多样性。结果显示,共得到可操作分类单元(operational taxonomic units,OTU)6 695个,分属46个菌门,421个菌属,在门水平上优势菌门为变形菌门(Proteobacteria),亚优势菌门为酸杆菌门(Acidobacteriota)和厚壁菌门(Firmicutes),变形菌门在各样本中的含量占比不同,但都属于优势菌群。在属水平上优势菌属为梭菌属(Clostridium),亚优势菌属为苔藓杆菌属(Bryobacter)和玫瑰螺菌属(Roseiarcus)。金线莲土壤6个样本共有OTUs数938个,占各样本OTUs数大于80.00%。金线莲根际土壤微生物的丰富度和均匀度高,物种组成较为相似。该研究为金线莲仿野生栽培的土壤改良奠定基础。

In order to analyze the species and abundance of soil microorganisms of wild Anoectochilus formosanus,optimize the soil conditions of Anoectochilus formosanus ecological cultivation,in this study,the high-throughput sequencing technology was used to perform on the 16S region library of soil microorganisms of Anoectochilus formosanus in 6 samples.The results showed that totally 6 695 operational taxonomic units(OTUs)were obtained,and assigned to 46 phylum and 421 genus with the dominant phylum of Proteobacteria and the subdominant phylum of Acidobacteria and Firmicutes.The proportion of Proteobacteria in each sample was varied,but that were belonged to dominant phylum.The dominant genus was Clostridium,and the subdominant genus was Bryobacter and Roseiarcus.The number of common bacterial OTUs Anoectochilus formosanus soil samples was938,the number of OTUs in each sample was more than 80.00%.The richness and uniformity of rhizosphere soil microorganisms were high,and the species composition was similar.This study laid a foundation for soil improvement of Anoectochilus formosanus imitation wild cultivation.