用于生物检测的过氧化氢荧光探针研究进展

Research progress of hydrogen peroxide fluorescence probes for biological detection

过氧化氢(H_(2)O_(2))是活性氧分子中重要的一种,人体正常水平的H_(2)O_(2)不会对机体造成损害。但是,细胞内过氧化氢的持续过度产生可能会导致细胞和生物分子的氧化损伤,引发生物体多种功能障碍,导致多种疾病的发生。因此,可视化、动态、实时地靶向和准确地定量细胞中的H_(2)O_(2),对于了解其在细胞生理学中的作用进而用于病理诊断的标志物具有至关重要的作用。生物兼容性高的H_(2)O_(2)荧光探针可以对生物样本进行无损分析,具有广泛的应用前景。近年来,多种不同的H_(2)O_(2)荧光探针在相关生物学事件中的应用显著促进H_(2)O_(2)探针在医学和生物学领域的发展。该文介绍近年用于H_(2)O_(2)生物检测的有机探针及无机纳米探针两种荧光探针的研究机理及应用现状,并对探针的存在问题及研究方向进行展望,为进一步进行该领域的研究提供理论支撑。

Hydrogen peroxide(H_(2)O_(2)),as an important molecule of reactive oxygen species,normal levels of which in the human body will not cause damage to the body.However,the continuous excessive production of hydrogen peroxide within cells may lead to oxidative damage to cells or biomolecules,various dysfunction of organisms,and the occurrence of many diseases.Therefore,visualization,dynamic,real-time targeting,and accurate quantification of H_(2)O_(2)in cells are crucial for understanding its role in cell physiology and serving as biomarkers for pathological diagnosis.H_(2)O_(2)fluorescent probes with high biocompatibility can perform nondestructive analysis of biological samples and have broad application prospects.In recent years,the application of various H_(2)O_(2)fluorescent probes in related biological events has significantly promoted the development of H_(2)O_(2)probes in the fields of medicine and biology.This article introduces the research mechanism and application advantages of organic probe and inorganic nanoprobe,used for H_(2)O_(2)biological monitoring in recent years,and prospects the existing problems and research directions of the probes,providing theoretical support for further research in this field.