光周期和维生素对巴夫藻生长和物质生产能力的影响

Effects of Photoperiod and Vitamin on Growth and Material Production Capacity of Pavlova sp.

【目的】研究光周期和维生素对巴夫藻(Pavlova sp.)生长和物质产量的影响,为提高巴夫藻的培养效益提供实验依据。【方法】采用维生素浓度和光周期的双因素实验,培养巴夫藻14 d,测定藻细胞中色素、脂肪、蛋白质和多糖的含量,并分析脂肪酸组成和生物柴油性能的变化。【结果】最适合巴夫藻生长的光周期是16 h∶8 h。在1V 16 h∶8 h的条件下,巴夫藻的生物量干质量和蛋白质产量最高,分别为501.50和55.54 mg·L^(-1)。在8V 8 h∶16 h条件下,岩藻黄素含量和产量最高,分别为6.21 mg·g^(-1)和2.60 mg·L^(-1)。在8V 24 h∶0 h和8V 16 h∶8 h条件下,脂肪含量和产量最高,分别为30.18%和123.87 mg·L^(-1)。最适合二十碳五烯酸(Eicosapentaenoic acid,EPA)生产的光周期为8 h∶16 h,在1V 8 h∶16 h的条件下,EPA占比达15.46%。在4V 24 h∶0 h条件下,生物柴油的十六烷值(CN)达到53.98,不饱和度(DU)和碘值(IV)最低。【结论】与维生素浓度相比,光周期对巴夫藻培养更为重要,光周期不仅影响巴夫藻的生长,还对其物质生产影响显著,并能改变巴夫藻的脂肪酸组成和生物柴油性能。

【Objective】To explore the effects of photoperiod and vitamin concentration on the growth and material production of Pavlova sp.,in order to provide the experimental basis for determining the optimal culture conditions of Pavlova sp.【Method】The two-factor experiment of vitamin concentration and photoperiod was conducted by culturing Pavlova sp.for 14 days.The contents of pigment,lipid,protein and carbohydrate in the alga cells were measured and the fatty acid composition and biodiesel performance were analyzed.【Result】The optimal photoperiod for the growth of Pavlova sp.was 16 h∶8 h.Under the condition of 1V 16 h∶8 h.The biomass dry weight and protein production of Pavlova sp.were the highest,which were 501.50 and 55.54 mg·L^(-1),respectively.Under the condition of 8V 8 h∶16 h,fucoxanthin content and production were the highest,which were 6.21 mg·g^(-1) and 2.60 mg·L^(-1),respectively.The highest lipid content and production were 30.18%and 123.87 mg·L^(-1) under 8V 24 h∶0 h and 8V 16 h∶8 h,respectively.The optimal photoperiod for EPA production was 8h∶16h.Under the condition of 1V 8 h∶16 h,the proportion of eicosapentaenoic acid(EPA)reached 14.56%.Under the condition of 4V 24 h∶0 h,the cetane number(CN)of biodiesel reached 53.98,while the degree of unsaturation(DU)and iodine value(IV)were the lowest.【Conclusion】Compared with vitamin concentration,photoperiod was more important for the culture of Pavlova sp..Photoperiod not only affected the growth of Pavlova sp.,but also significantly affected its material production,and could change the fatty acid composition and biodiesel performance of Pavlova sp.