镰刀菌YL2产纤维素酶液体发酵工艺优化

Response Surface Methodology-Based Optimization of Liquid Fermentation Process for Cellulase Production by Fusarium sp.YL2

为提高镰刀菌YL2发酵产纤维素酶的效率,采用Plackett-Burman试验设计和响应面法设计优化了镰刀菌YL2产纤维素酶的发酵工艺,确定该菌种的最适产酶培养基及最优产酶条件。结果表明:最佳培养基配方为稻草粉31.6 g/L、豆饼粉25 g/L、柠檬酸氢二铵2.0 g/L、硫酸镁1.0 g/L、蔗糖酯1.45 g/L,在发酵培养基初始pH值6.4、500 mL的三角瓶中装液量为80 mL、摇床转速225 r/min、接种量3%、培养温度30℃的最优产酶条件下培养6 d,微晶纤维素酶(AVI)和羧甲基纤维素酶(CMC)酶活为分别6.72和84.36 IU/mL。

Plackett-Burman design and response surface methodology were employed to optimize the fermentation conditions of Fusarium sp.YL2 for cellulase production.The results showed that the optimal medium was composed of 31.6 g/L straw powder,25 g/L soybean meal powder,2.0 g/L diammonium hydrogen citrate,1.0 g/L magnesium sulfate,and 1.45 g/L sucrose ester.The optimal fermentation conditions were initial pH 6.4,liquid volume of 80 mL in a 500-mL flask,inoculation amount of 3%,and incubation at 30℃with shaking at 225 r/min for 6 d.The fermentation liquid obtained under the optimal conditions showed the micro-crystalline cellulase(AVI)activity of 6.72 IU/mL and the carboxymethyl cellulase(CMC)activity of 84.36 IU/mL.